Methylscopolamine (bromide) - CAS:155-41-9

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Methylscopolamine (bromide) (Synonyms:甲基溴东莨菪碱; (-)-Scopolamine methyl bromide; Hyoscine methyl bromide)

目录号 : KCM11670 CAS No. : 155-41-9 纯度 : ≥98%

Data Sheet SDS 产品使用指南

Methylscopolamine is an antagonist of muscarinic acetylcholine receptors (Ks = 0.25, 0.37, 0.23, 0.22, and 0.3 nM for M respectively). It inhibits oxotremorine-induced gastric ulcer formation and pupillary light reflex in rats (EDs = 0.4 and 3.6 μg/kg, respectively). It also increases pupil diameter in rats with an ED value of 1.8 μg/kg. Formulations containing methylscopolamine have been used for the treatment of peptic ulcers, intestinal spasms, and motion sickness.

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500mg		In-stock
1g		In-stock

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生物活性

Methylscopolamine (bromide) is a peripherally acting muscarinic receptor (mAChR) antagonist that cannot cross the blood-brain barrier. Methscopolamine bromide blocks the muscarinic negative feedback regulation of acetylcholine release from striatal cholinergic terminals, thereby increasing acetylcholine release in the striatum of freely moving rats. Methscopolamine bromide does not induce motor excitation in freely moving rats, nor does it alter the duration of ethanol-induced loss of righting reflex in mice. Methscopolamine bromide fails to antagonize the arecoline-mediated reduction in the duration of ethanol-induced loss of righting reflex in mice.

体内研究

Methscopolamine (0.25-1.0 mg/kg; subcutaneous injection; Due to the inability to cross the blood-brain barrier, Arecoline cannot counteract the shortening effect of ethanol induced hypnosis duration in mice.
Methscopolamine bromamide (5-10 mg/kg; intraperitoneal injection; Single dose administration can increase the maximum release of acetylcholine in the striatum of freely moving rats by 380% and 430% respectively, without altering their autonomous motor activity.

Animal Model:	ICR mice (male, 18 to 22 g)
Dosage:	0.25 mg/kg; 1.0 mg/kg
Administration:	s.c.; single dose
Result:	Did not significantly alter the duration of ethanol-induced loss of the righting reflex (LORR) compared to saline-treated controls when administered alone. Did not reverse the arecoline-induced shortening of ethanol-induced LORR duration (no significant difference compared to the saline + arecoline group) when administered before arecoline.

Animal Model:	Wistar rats (male, 280-350 g)
Dosage:	5 mg/kg; 10 mg/kg
Administration:	i.p.; single injection
Result:	Caused a maximal 380% increase in striatal acetylcholine release relative to baseline levels at 2 hours post-injection, with release remaining above baseline for over 4 hours. Caused a maximal 430% increase in striatal acetylcholine release relative to baseline levels at 2 hours post-injection, with release remaining above baseline for over 4 hours. Did not cause a change in spontaneous motor activity relative to controls.

分子式

C₁₈H₂₄NO₄^.Br

分子量

398.29

CAS号

155-41-9

中文名称

甲溴东莨菪碱

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

DMSO : 50 mg/mL (125.54 mM; ultrasonic and warming and heat to 60°C)

H2O : 50 mg/mL (125.54 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.5107 mL	12.5537 mL	25.1073 mL
5 mM	0.5021 mL	2.5107 mL	5.0215 mL
10 mM	0.2511 mL	1.2554 mL	2.5107 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO → 40% PEG300 → 5% Tween-80 → 45% saline

Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.28 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO → 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.28 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
3.

请依序添加每种溶剂： 10% DMSO → 90% corn oil

Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.28 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

纯度： ≥98%

Data Sheet

SDS

产品使用指南

Sun YP, et al. Systemic administration of arecoline reduces ethanol-induced sleeping through activation of central muscarinic receptor in mice. Alcohol Clin Exp Res. 2010;34(1):150-157.

Watanabe H, et al. Effect of anticholinergic drugs on striatal acetylcholine release and motor activity in freely moving rats studied by brain microdialysis. Jpn J Pharmacol. 1989;51(1):75-82.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

Other Forms of Rapamycin:

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器