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Cell Cycle/DNA Damage——DNA/RNA Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cell Cycle/DNA Damage——DNA/RNA Synthesis(249)

Cat. No. Product Name CAS No. Purity Chemical Structure
KM4617 (+)-TK216

(+)-TK216 是 TK216 的对映异构体。TK216 是一种口服有效的 E26 转录因子 (ETS) 抑制剂。

1903783-77-6 99%
KM4616 (-)-TK216

(-)-TK216 是 TK216 的对映异构体。TK216 是一种口服有效的 E26 转录因子 (ETS) 抑制剂。(-)-TK216 具有抗癌作用。

1903783-78-7 99%
KM3787 (S)-Crizotinib

(S)-Crizotinib 是一种有效的选择性 MTH1 (mutT 同源物)抑制剂,IC50 为 330 nM。(S)-Crizotinib 通过抑制 MTH1 破坏核苷酸库的稳态,诱导 DNA 单链断裂的增加,激活人结肠癌细胞的 DNA 修复,并有效抑制动物模型中的肿瘤生长。

1374356-45-2 98%
KE6569 1,6,7-Trihydroxyxanthone 25577-04-2 ≥95%
KE4196 1-Decarboxy-3-oxo-ceanothic acid 214150-74-0 ≥95%
KM16716 1-Hydroxyanthraquinone

1-Hydroxyanthraquinone 是具有口服活性的天然产物,可来源于一些植物如 Tabebuia avellanedae,具有致癌作用。

129-43-1 98%
KM7957 2'-O-Me-C(Bz) Phosphoramidite

2'-O-Me-C(Bz) Phosphoramidite 是一种修饰过的亚磷酰胺单体,可用于寡核苷酸的合成。

110764-78-8
KM10975 2'-OMe-A(Bz) Phosphoramidite

2'-OMe-A(Bz) Phosphoramidite 是一种修饰过的亚磷酰胺单体,可用于寡核苷酸的合成。

110782-31-5 98%
KM11136 2'-OMe-G(ibu) Phosphoramidite

2'-OMe-G(ibu) Phosphoramidite 是一种修饰过的亚磷酰胺单体,可用于寡核苷酸的合成。

150780-67-9
KM17805 2,4-D

2,4-D (2,4-Dichlorophenoxyacetic acid) 是一种用于阔叶杂草防治的选择性除草剂。2,4-D 作为一种植物激素,在分生组织中引起不受控制的生长。2,4-D 抑制 DNA 和蛋白质的合成,从而阻止植物的正常生长发育。

94-75-7 ≥97%
KM5786 2-(Methylamino)-1H-purin-6(7H)-one

2-(Methylamino)-1H-purin-6(7H)-one (N2-Methylguanine) 是经过修饰的核苷,是在人体液中存在的内源性甲基化核苷。

10030-78-1 ≥98%
KE7356 2-Keto-D-galactose 54142-77-7 ≥95%
KM15092 2-O-Methylcytosine

2-O-Methylcytosine,一种 O-烷基化类似物和 DNA 的加合物,属于受损的核碱基。

3289-47-2 ≥97%
KM11373 3'-Deoxyuridine-5'-triphosphate

3'-Deoxyuridine-5'-triphosphate (3'-dUTP) 是一种核苷酸类似物,可抑制 DNA 依赖性 RNA 聚合酶 I 和 II (RNA polymerases III),强烈并竞争性地抑制 UTP 掺入到 RNA 中,Ki 值为 2.0 μM。

69199-40-2
KM7726 3'-Deoxyuridine-5'-triphosphate trisodium

3'-Deoxyuridine-5'-triphosphate trisodium (3'-dUTP trisodium) 是一种核苷酸类似物,可抑制 DNA 依赖性 RNA 聚合酶 I 和 II (RNA polymerases III),强烈并竞争性地抑制 UTP 掺入到 RNA 中,Ki 值为 2.0 μM。

99%
KM14027 3,4-Dihydroxybenzylamine hydrobromide

3,4-Dihydroxybenzylamine hydrobromide (NSC 263475 hydrobromide) 是一种结构改良的多巴胺类似物细胞毒物,抑制黑色素瘤细胞中 DNA 聚合酶 (DNA polymerase) 活性。3,4-Dihydroxybenzylamine hydrobromide 在不同程度的酪氨酸酶活性的黑色素瘤细胞系中显示不同的生长抑制活性。

16290-26-9 ≥95%
KM5353 3-AP

3-AP (PAN-811) 是一种核糖核苷酸还原酶 (ribonucleotide reductaseRR) 的 M2 亚基抑制剂,是有效的放射致敏剂。

143621-35-6 99%
KM15919 3-Isomangostin

3-Isomangostin 可从 Garciniamangostana.L. 壳中提取,3-Isomangostin 是一种有效的 MutT 同系物 1 (MTH1) 抑制剂,IC50 值为 52 nM。3-Isomangostin 可用于进行开发抗癌剂。

19275-46-8
KM16590 4-Methoxyphenethyl alcohol

4-Methoxyphenethyl alcohol,一种芳香族醇,是由 A. albispathus Hett. 产生的茴香样气味中的主要成分。4-Methoxyphenethyl alcohol 可以抑制大肠杆菌中的蛋白质,RNA 和 DNA 合成。

702-23-8
KM6733 5'-O-DMT-2'-O-TBDMS-Ac-rC

5'-O-DMT-2'-O-TBDMS-Ac-rC 是一种修饰的核苷,可以用于合成 DNA 或 RNA。

121058-85-3
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