2-(Methylamino)-1H-purin-6(7H)-one (N2-Methylguanine) is a modified nucleoside. 2-(Methylamino)-1H-purin-6(7H)-one is an endogenous methylated nucleoside found in human fluids.
IC50&Target
Human Endogenous Metabolite
体外研究
2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine) is found within both helical and looped regions of RNA secondary structure, and it can exist in either the s-cis or the s-trans rotamer. If there is a rotational preference for the methyl group, the effect of 2-(Methylamino)-1H-purin-6(7H)-one substitution may be specific to the sequence context depending upon which face of the base participates in hydrogen bonding. 2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine) is the principal kinetic barrier for reverse transcription in the 1340 bases proximal to the 5' end of E. coli 16S rRNA. Transcription intermediates correspond to attenuation at the positions of 2-(Methylamino)-1H-purin-6(7H)-one in the rRNA sequence. The relaxation time for elongation of the cDNA through m2G is approximately 3 min.
分子式
C6H7N5O
分子量
165.15
CAS号
10030-78-1
中文名称
N2-甲基鸟嘌呤
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
溶解性数据
In Vitro:
DMSO : 1 mg/mL (6.06 mM; Need ultrasonic)
H2O : 1 mg/mL (6.06 mM; ultrasonic and warming and heat to 80°C)
Ethanol : < 1 mg/mL (ultrasonic) (insoluble)
DMF : < 1 mg/mL (ultrasonic) (insoluble)
1M NaOH : < 1 mg/mL (ultrasonic;adjust pH to 12 with NaOH) (insoluble)
Acetone : < 1 mg/mL (ultrasonic) (insoluble)
THF : < 1 mg/mL (ultrasonic) (insoluble)
0.1 M HCL : < 1 mg/mL (ultrasonic;adjust pH to 2 with HCl) (insoluble)