Sodium chloride, for cell culture (Synonyms:Halite, for cell culture;氯化钠)
目录号 : KP18982 CAS No. : 7647-14-5 纯度 : 99%
Sodium chloride, for cell culture 是一种具有口服活性的盐类。它能够诱导 ATP1A1 的表达,并促进促炎细胞因子 IL-2、TNFα、IL-9 及多种趋化因子的表达。此外,该化合物可增强 Digoxin 对小细胞肺癌的抗肿瘤活性,还能通过诱导致病性 Th17 细胞来驱动自身免疫性疾病。
规格 价格 是否有货 数量
100g
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生物活性

Sodium chloride, for cell culture is an orally active salt. Sodium chloride, for cell culture induces the expression of ATP1A1. Sodium chloride, for cell culture induces the pro-inflammatory cytokines IL-2, TNFα, IL-9 and several chemokines. Sodium chloride, for cell culture enhances the anti-tumor activities of Digoxin against small cell lung cancer. Sodium chloride, for cell culture drives autoimmune disease by the induction of pathogenic Th17 cells.

IC50&Target

IL-2                                        IL-9

体外研究

Sodium chloride (10-70 mM; 72 h) can enhance the anti-tumor activity of Digoxin in small cell lung cancer cell lines.
Sodium chloride (40 mM) can specifically promote the generation of highly pathogenic Th17 cells.

体内研究

Sodium chloride (3%; oral and intravenous injection; Administration of Digoxin via drinking water and intravenous injection of 200 μ L of 3% NaCl 6 hours prior to treatment can enhance the anti-tumor activity of Digoxin in a mouse model of small cell lung cancer.
Sodium chloride (1% -4%; oral; Feed containing 4% NaCl and drinking water containing 1% NaCl can induce Th17 cells and exacerbate experimental autoimmune encephalomyelitis in mice.

 

Animal Model: Small cell lung cancer mice
Dosage: 3%
Administration:
p.o. and i.v.; given by drinking water for 6 h before treatment and i.v. with 200 μL 3% NaCl
Result: Did not induce significant tumor growth inhibition.
Reduced tumor volume when combination with Digoxin.
 
分子式
ClNa
分子量
58.44
CAS号
7647-14-5
中文名称
氯化钠
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
keep dry and cool
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶解性数据
In Vitro: 

H2O : 200 mg/mL (3422.31 mM; Need ultrasonic)

配制储备液
                                           
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
                                                                                                                    
1 mM 17.1116 mL 85.5578 mL 171.1157 mL
5 mM 3.4223 mL 17.1116 mL 34.2231 mL
10 mM 1.7112 mL 8.5558 mL 17.1116 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
动物实验计算换算器
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系客服为您提供正确的澄清溶液配方)
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计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,

体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。

配置后的溶液总体积:

1. 首先保证母液是澄清的;
           2. 一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。