Sulfo-NHS-Biotin是一种水溶性生物素化试剂，用于简单高效地标记抗体、蛋白质和含伯胺的分子。生物素是一种维生素，能与阿维丁和链霉亲和素蛋白高度亲和结合。生物素很小（244 Da），可与蛋白质结合而不改变其生物活性。在 pH 值为 7-9 的缓冲液中，NHS 活化的生物素可与伯氨基（-NH2）结合形成稳定的酰胺键。Sulpho NHS 生物素还可用于标记膜蛋白；由于琥珀酰亚胺环上的亚硫酸钠基团带有电荷，它无法渗透细胞膜。如果细胞保持完整，则只有暴露在表面的伯胺会被生物素标记。
| 生物活性 |
Sulfo-NHS-Biotinis a water soluble biotinylation reagent used for simple and efficient labeling of antibodies, proteins and primary amine-containing molecules. Biotin is a vitamin which binds with high affinity to avidin and streptavidin proteins. Biotin is small (244 Da), and may be conjugated to proteins without altering their biological activities. NHS-activated biotins couple to primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. Sulpho NHS biotin has also been used for labeling of membrane proteins; due to the charge of the sodium sulfoxide group on the succinimidyl ring, it cannot permeate the cell membrane. If the cell remains intact, only primary amines exposed on the surface will be biotinylated. |
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| 分子式 |
C14H19N3O7S2
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| 分子量 |
405.45
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| CAS号 |
119616-38-5
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| 中文名称 |
生物素琥珀酰亚胺酯
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| 运输条件 |
Room temperature in continental US; may vary elsewhere. |
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| 储存方式 |
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