Calcein Blue AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Blue AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Blue by intracellular esterases. Calcein Blue is retained by living cells and displays excitation/emission maxima of 360/445 nm, respectively. Calcein Blue AM is a blue-emitting variant of the green fluorophore Calcein AM , which has been used to evaluate cell viability and cytotoxicity.
Calcein Blue AM is a cell staining agent. Calcein Blue AM serves as a substrate for intracellular esterases, which hydrolyze its acetoxymethyl (AM) ester group to convert the non-fluorescent reagent into fluorescent Calcein Blue. Calcein Blue AM passively penetrates the plasma membrane of both live and dead cells; it is converted into a polar fluorescent product and retained in the cytoplasm of live cells, whereas dead cells cannot retain the dye. Calcein Blue AM exhibits significant dye leakage, and its fluorescence intensity gradually decreases over time. Calcein Blue AM can be used for endpoint-based cell viability assays and short-term cell tracking experiments with a duration of no more than 4 hours.
体外研究
Calcein Blue AM can serve as a substrate for cell lactonases, and esterases hydrolyze its acetoxymethyl (AM) ester group, converting non fluorescent reagents into Calcein Blue with fluorescence.
分子式
C21H23NO11
分子量
465.41
CAS号
168482-84-6
中文名称
Calcein blue, AM
运输条件
Room temperature in continental US; may vary elsewhere.