PicoGreen是一种特异性结合双链DNA (dsDNA) 的饱和荧光染料，激发波长为480 nm，发射波长为520 nm，与大多数荧光计和荧光酶标仪兼容。 PicoGreen仅与dsDNA结合后才发出荧光， 不受核苷酸和单链核酸的影响， 产生的荧光强度与dsDNA浓度成正比， 且无序列依赖性， 适合于dsDNA的定量检测， 可检出25 pg/ml~1 μg/ml浓度范围内的dsDNA。
| 生物活性 |
PicoGreen is a saturable fluorescent dye that specifically binds to double-stranded DNA (dsDNA), with an excitation wavelength of 480 nm and an emission wavelength of 520 nm. It is compatible with most fluorometers and fluorescence microplate readers. PicoGreen fluoresces only after binding to dsDNA and is unaffected by nucleotides or single-stranded nucleic acids. The resulting fluorescence intensity is directly proportional to the concentration of dsDNA and exhibits no sequence dependence, making it suitable for quantitative detection of dsDNA. It can detect dsDNA concentrations ranging from 25 pg/ml to 1 μg/ml.
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|---|---|
| 分子式 |
C₃₄H₄₂ClN₅S
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| 分子量 |
588.26
|
| 运输条件 |
Room temperature in continental US; may vary elsewhere.
|
| 储存方式 |
2-8°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
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