Protonex™ Red 670, NHS ester 荧光强度会随环境pH值动态变化：在碱性条件下荧光信号极弱，而在酸性环境中荧光强度达到最大值。当该染料与细胞表面受体或抗体结合时，由于细胞外中性pH环境，染料保持非荧光状态。过内体途径转运至酸性环境（pH降低），染料立即被激活，红色激光激发下发出强烈红色荧光。(Ex/Em=643/660nm)
| 生物活性 |
Protonex™ Red 670, NHS ester The fluorescence intensity will dynamically change with the environmental pH value: the fluorescence signal is extremely weak under alkaline conditions, while the fluorescence intensity reaches its maximum value in acidic environments. When the dye binds to cell surface receptors or antibodies, it remains in a non fluorescent state due to the neutral pH environment outside the cell. The dye is immediately activated when transported to an acidic environment (pH decreases) through the endosomal pathway, and emits strong red fluorescence under red laser excitation. (Ex/Em=643/660nm) |
|---|---|
| 分子量 |
827.02
|
| 运输条件 |
Room temperature in continental US; may vary elsewhere.
|
| 储存方式 |
Store at below -15°C, protected from light. |
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