Potassiumalum, for cell culture - CAS:7784-24-9

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Potassiumalum, for cell culture (Synonyms:十二水合硫酸铝钾,用于细胞培养；钾明矾,用于细胞培养;Aluminum potassium sulfate dodecahydrate, for cell culture)

目录号 : KM32714 CAS No. : 7784-24-9 纯度 : 98%

Data Sheet 产品使用指南

Potassiumalum, for cell culture 是一种卵清蛋白佐剂。Potassiumalum, for cell culture 可作为媒染剂帮助染料结合组织成分，增强染色效果及染色过程中的稳定性。Potassiumalum, for cell culture 可诱导小鼠产生过敏反应。Potassium alum, for cell culture 可用于细菌和组织染色相关研究。

规格	价格	是否有货	数量
5g		In-stock
10g		In-stock
25g		In-stock
50g		In-stock

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Potassiumalum, for cell culture In-stock

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生物活性

Potassiumalum, for cell culture is an egg white adjuvant. Potassiumalum, for cell culture acts as a mordant to help dyes bind to tissue components, enhancing the staining effect and stability during staining. Potassiumalum, for cell culture can induce allergic reactions in mice. Potassium alum, for cell culture can be used in research for bacterial and tissue staining.

体外研究

Potassium irreversibly inhibits the activities of α - amylase (70% decrease in activity), protease (51% decrease in activity), and lipase (31% decrease in activity) in the midgut extract of adult American cockroach (Periplaneta Americana) for 4 hours, and has a stronger inhibitory effect on females than males.
Potassium can be used as an adjuvant for ovalbumin to induce allergic reactions in mice.

体内研究

The standardized Vibrio anguillarum vaccine supplemented with 2.5% Potassium (intraperitoneal injection, 50-100 μL) as an adjuvant can significantly inhibit the growth of rainbow trout, causing severe chronic peritonitis and organ damage.
The standardized inactivated vaccine against Vibrio anguillarum, supplemented with 2.5% Potassiumalum (intraperitoneal injection, 100-200 μL) as an adjuvant, resulted in extremely low mortality rate, significantly slowed growth, and mild to moderate organ damage in rainbow trout.

Animal Model:

Salmo gairdneri Richardson (approximately 3 cm length, mean weight 1.5 g)

Dosage:

50; 100 μl (adjuvanted with 2.5% Potassiumalum)

Administration:

i.p.

Result:

Reached 67% cumulative mortality at 48 days post-vaccination for the 50 μl adjuvanted dose.
Reached 74% cumulative mortality at 48 days post-vaccination for the 100 μl adjuvanted dose.
Achieved 231% weight increase over six weeks for the 50 μl adjuvanted dose.
Caused severe reactive peritonitis, inflammatory oedema of visceral peritoneum, macrophage infiltration and adjuvant deposition in serosa of ovary, liver, and pancreas, progressive focal degeneration of pancreatic acini, granulomata formation, fibroblast proliferation with collagen deposition, splenic and renal haemopoietic tissue depletion, occasional subcapsular hepatic necrosis, and heavy sequestration of alum into hepatocytes.
Showed no significant additional protection against Vibrio anguillarum challenge compared to non-adjuvanted vaccine.

Animal Model:	Salmo gairdneri Richardson (20 cm length, mean weight 100 g-156 g)
Dosage:	100; 200 μl (adjuvanted with 2.5% Potassiumalum)
Administration:	i.p.
Result:	Reached 5% cumulative mortality at 48 days post-vaccination for the 100 μl adjuvanted dose. Reached 6% cumulative mortality at 48 days post-vaccination for the 200 μl adjuvanted dose. Achieved 53% weight increase over six weeks for the 100 μl adjuvanted dose. Achieved 26% weight increase over six weeks for the 200 μl adjuvanted dose. Caused peritoneal oedema, macrophage infiltration, distortion of pancreatic acini, focal hepatic necrosis, severe focal necrosis of renal haemopoietic tissue, sequestration of alum into hepatocytes, pancreatic tissue fibrosis, organizing focal necrosis of liver and kidney, increased macrophage activity in peritoneal tissues, reduction in splenic white pulp, and gonad damage with fibrosed tissue contraction by one month post-injection. Showed no significant additional protection against Vibrio anguillarum challenge compared to non-adjuvanted vaccine.

分子式

AlH₂₄KO₂₀S₂

分子量

474.39

CAS号

7784-24-9

中文名称

十二水合硫酸铝钾,用于细胞培养；钾明矾,用于细胞培养

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H2O: 200 mg/mL (421.59 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.1080 mL	10.5399 mL	21.0797 mL
5 mM	0.4216 mL	2.1080 mL	4.2159 mL
10 mM	0.2108 mL	1.0540 mL	2.1080 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

纯度： 98%

Data Sheet

产品使用指南

Horne M T, et al. The effects of the use of potassium alum adjuvant in vaccines against vibriosis in rainbow trout, Salmo gairdneri Richardson. J. Fish Dis. 1984, 7, 91-99.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

Other Forms of Rapamycin:

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器