Acetyl-L-carnitine - CAS:3040-38-8

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Acetyl-L-carnitine (Synonyms:L-乙酰基肉碱;O-Acetyl-L-carnitine; ALCAR)

目录号 : KM32534 CAS No. : 3040-38-8 纯度 : 99%

Data Sheet 产品使用指南

Acetyl-L-carnitine (O-Acetyl-L-carnitine; ALCAR) 是一种口服有效的线粒体能量代谢调节剂及神经保护剂，可透过血脑屏障。Acetyl-L-carnitine 通过有机阳离子转运体 OCTN2 选择性进入细胞及大脑。Acetyl-L-carnitine 可作为乙酰供体参与脂肪酸 β-氧化、促进乙酰胆碱合成、调节线粒体功能及抑制氧化应激。Acetyl-L-carnitine 通过提升能量代谢、保护神经元和改善突触可塑性发挥活性。Acetyl-L-carnitine 主要用于新生儿缺氧缺血性脑损伤、阿尔茨海默病、抑郁症等神经退行性疾病及代谢紊乱相关疾病的研究。

规格	价格	是否有货	数量
10mg		In-stock
25mg		In-stock
50mg		In-stock
100mg		In-stock
500mg		In-stock
10 mM * 1 mL in DMSO		In-stock

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生物活性

Acetyl-L-carnitine (O-Acetyl-L-carnitine; ALCAR) is an orally active mitochondrial energy metabolism regulator and neuroprotectant that can penetrate the blood-brain barrier. Acetyl-L-carnitine selectively enters cells and the brain through the organic cation transporter OCTN2. Acetyl-L-carnitine can participate in fatty acid β-oxidation, promote acetylcholine synthesis, regulate mitochondrial function and inhibit oxidative stress as an acetyl donor. Acetyl-L-carnitine exerts its activity by enhancing energy metabolism, protecting neurons and improving synaptic plasticity. Acetyl-L-carnitine is mainly used in the study of neurodegenerative diseases and metabolic disorder-related diseases such as neonatal hypoxic-ischemic brain damage, Alzheimer's disease, and depression.

IC₅₀&Target

Human Endogenous Metabolite

体外研究

Cell viability and apoptosis:
Acetyl-L-carnitine (1 mM; 30 min) significantly inhibited caspase-3/9 activation induced by 1 μ g/mL Doxycycline in DT40 cells with thioredoxin TRX2 deficiency, reduced reactive oxygen species (ROS) production and cell apoptosis, and maintained cysteine levels [1].

Cell Viability Assay

Cell Line:	TRX2-/- DT40 cells (chicken B-cell line)
Concentration:	1 mM
Incubation Time:	30 min pretreatment before 1 μg/mL Doxycycline induction
Result:	Reduced activation of caspase-3 and caspase-9, key markers of mitochondrial apoptosis pathway, compared to untreated cells. Decrease 40% in intracellular ROS levels, indicating attenuation of oxidative stress. Maintained glutathione levels in both cytosol and mitochondria, preventing redox imbalance caused by TRX2 deficiency. Resulted 35% reduction in apoptotic cell population, confirming protective effects against mitochondrial-dependent apoptosis.

体内研究

Acetyl-L-carnitine (100 mg/kg; intraperitoneal injection; Every time; 14 days) significantly reduced brain damage in the neonatal hypoxic-ischemic brain injury (HIBD) model, improved neurological function, lowered oxidative stress levels, and reduced cell apoptosis [2].
Acetyl-L-carnitine (300 mg/kg; oral gavage; Every time; 28 days) Improve the cognitive function of the elderly cognitive impairment model, enhance synaptic plasticity in the hippocampus, and increase the expression of brain-derived neurotrophic factor (BDNF).

Animal Model:	Neonatal Sprague-Dawley rats (male, 18-22g, postnatal day 7), hypoxic-ischemic brain damage (HIBD) model[2]
Dosage:	100 mg/kg
Administration:	Intraperitoneal injection, once daily, for 14 consecutive days starting 24 hours after HIBD induction
Result:	Brain injury: Reduced infarct volume by 40% compared to the HIBD control group as measured by triphenyltetrazolium chloride (TTC) staining. Neurological function: Improved neurological scores, including motor function, sensory function, and balance, as evaluated by a modified neurological severity score (mNSS).

分子式

C₉H₁₇NO₄

分子量

203.24

CAS号

3040-38-8

中文名称

L-乙酰基肉碱；乙酰左卡尼汀

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, keep dry and cool

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

H2O : 100 mg/mL (492.03 mM; Need ultrasonic)

DMSO : 14.29 mg/mL (70.31 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.9203 mL	24.6015 mL	49.2029 mL
5 mM	0.9841 mL	4.9203 mL	9.8406 mL
10 mM	0.4920 mL	2.4601 mL	4.9203 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO → 40% PEG300 → 5% Tween-80 → 45% saline

Solubility: ≥ 2.5 mg/mL (12.30 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (12.30 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO → 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (12.30 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (12.30 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
3.

请依序添加每种溶剂： 10% DMSO → 90% corn oil

Solubility: ≥ 2.5 mg/mL (12.30 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (12.30 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

纯度： 99%

Data Sheet

产品使用指南

[1]. Zhu X, et al. Acetyl-L-carnitine suppresses apoptosis of thioredoxin 2-deficient DT40 cells. Arch Biochem Biophys. 2008 Oct 15;478(2):154-60.

[2]. Wang SM, et al. A review of current evidence for acetyl-l-carnitine in the treatment of depression. J Psychiatr Res. 2014 Jun;53:30-7.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

Other Forms of Rapamycin:

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器