BRD4770 - CAS:1374601-40-7

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BRD4770

目录号 : KM9996 CAS No. : 1374601-40-7 纯度 : 98%

Data Sheet 产品使用指南

BRD4770 是一种组蛋白甲基转移酶 G9a 抑制剂。BRD4770 可抑制 H3K9 的二甲基和三甲基化，EC₅₀ 为 5 µM，对 H3K27me3，H3K36me3，H3K4me3 和 H3K79me3 几乎没有影响。BRD4770 可以激活共济失调毛细血管扩张突变 (ATM) 途径并诱导细胞衰老。

规格	价格	是否有货
10mg		In-stock
50mg		In-stock
100mg		In-stock
500mg		In-stock
1g	询价	In-stock
5g	询价	In-stock

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生物活性

BRD4770 is a histone methyltransferase G9a inhibitor. BRD4770 reduces di- and trimethylation of lysine 9 on histone H3 (H3K9) with an EC₅₀ of 5 µM, and has less or little effect toward H3K27me3, H3K36me3, H3K4me3, and H3K79me3. BRD4770 can activate the ataxia telangiectasia mutated (ATM) pathway and induce cell senescence.

体外研究

BRD4770 (0-20 µM; 72 hours; PANC-1 cells) treatment reduces the number of cells after 72 h.
BRD4770 (2.5-5 µM; 24 hours; PANC-1 cells) treatment decreases H3K9 trimethylation level by 23% in PANC-1 cells.
BRD4770 induces a senescent phenotype in a pancreatic cancer cell line. BRD4770 also inhibits both anchorage-dependent and -independent cell proliferation and induces G2/M cell-cycle arrest. BRD4770 activates the ataxia telangiectasia mutated (ATM) pathway without inducing DNA damage, while the ataxia telangiectasia and Rad3-related protein (ATR) pathway is not affected.
BRD4770 also induces increased levels of lysine acetylation in cells without inhibiting histone deacetylases.

Cell Viability Assay

Cell Line:	PANC-1 cells
Concentration:	0 µM, 0.625 µM, 1.25 µM, 2.5 µM, 5 µM, 10 µM, 20 µM
Incubation Time:	72 hours
Result:	Reduced the number of cells after 72 h.

Western Blot Analysis

Cell Line:	PANC-1 cells
Concentration:	2.5 µM, 5 µM
Incubation Time:	24 hours
Result:	Decreased H3K9 trimethylation level by 23% in PANC-1 cells.

分子式

C₂₅H₂₃N₃O₃

分子量

413.47

CAS号

1374601-40-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 33.33 mg/mL (80.61 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4186 mL	12.0928 mL	24.1856 mL
5 mM	0.4837 mL	2.4186 mL	4.8371 mL
10 mM	0.2419 mL	1.2093 mL	2.4186 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 0.5% Methylcellulose/saline water
Solubility: 6.25 mg/mL (15.12 mM); Suspended solution; Need ultrasonic
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (6.05 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.05 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

科研文献

纯度： 98%

Data Sheet

产品使用指南

摩尔计算器
稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

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KKL 顾客使用本产品发表的 2 篇科研文献

The molarity calculator equation

The dilution calculator equation