AkaLumine hydrochloride - CAS:2558205-28-8

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AkaLumine hydrochloride

目录号 : KM9394 CAS No. : 2558205-28-8 纯度 : 95%

Data Sheet 产品使用指南

AkaLumine hydrochloride 是荧光素的一个类似物，其对重组荧光蛋白的 K_m 值为 2.06 μM。

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10mg		In-stock
50mg	询价	In-stock
100mg	询价	In-stock
200mg	询价	In-stock
500mg	询价	In-stock

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生物活性

AkaLumine hydrochloride is a luciferin analogue, with a K_m of 2.06 μM for recombinant Fluc protein.

体外研究

In both LLC/luc and MDA-MB-231/luc cells treated with AkaLumine hydrochloride, the signal is maximal at lower concentrations (by 2.5 μM), whereas the bioluminescence generated by D-luciferin and CycLuc1 increase as concentrations increase further, not appearing to reach a maximal signal even at 250 μM.

体内研究

AkaLumine hydrochloride displays greatly enhanced signals emanating from metastasis in the lung, as demonstrated by 8.1-fold higher with AkaLumine hydrochloride as compared with D-luciferin administration. To evaluate the advantage of AkaLumine hydrochloride over CycLuc1 in detecting targets in deep tissue, bioluminescence images are compared between AkaLumine hydrochloride- and CycLuc1-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, which is the maximum concentration of CycLuc1 due to its low water solubility. AkaLumine hydrochloride exhibits 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with CycLuc1. The advantage of AkaLumine hydrochloride is further confirmed by imaging the same mice bearing lung metastasis after intraperitoneal injection of 5 mM substrates in order of CycLuc1 and AkaLumine hydrochloride, and in the inverse order at an 8-h interval. AkaLumine hydrochloride displays about fourfold increase in signals from lung metastasis compared with CycLuc1.

分子式

C₁₆H₁₉ClN₂O₂S

分子量

338.85

CAS号

2558205-28-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, stored under nitrogen

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

溶解性数据

In Vitro:

DMSO : 100 mg/mL (295.12 mM; Need ultrasonic)

H₂O : 50 mg/mL (147.56 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9512 mL	14.7558 mL	29.5116 mL
5 mM	0.5902 mL	2.9512 mL	5.9023 mL
10 mM	0.2951 mL	1.4756 mL	2.9512 mL

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO → 40% PEG300 → 5% Tween-80 →45% saline

Solubility: ≥ 4 mg/mL (11.80 mM); Clear solution

此方案可获得 ≥ 4 mg/mL (11.80 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 40.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO → 90% (20% SBE-β-CD in saline)

Solubility: ≥ 4 mg/mL (11.80 mM); Clear solution

此方案可获得 ≥ 4 mg/mL (11.80 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 40.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。