Batabulin (T138067; 30-300 nM; 24 hours; MCF7 cells) treatment shows approximately 25-30% tetraploid (4n) DNA content in cells, indicating an arrest at the G2/M cell-cycle boundary.
Batabulin (T138067; 30-300 nM; 24-48 hours; MCF7 cells) treatment shows 25-30% apoptosis. After a 48-hr exposure to 100 nM Batabulin, approximately 50-80% of the cell population is undergoing apoptosis.
Batabulin (T138067) binds covalently and selectively to a subset of the β-tubulin isotypes, thereby disrupting microtubule polymerization. Covalent modification occurs at a conserved Cys-239 shared by the β1, β2, and β4 tubulin isotypes. Cells exposed to Batabulin become altered in shape, indicating a collapse of the cytoskeleton, and show an increase in chromosomal ploidy.
Cell Cycle Analysis
Cell Line: |
MCF7 cells |
Concentration: |
30 nM, 100 nM and 300 nM |
Incubation Time: |
24 hours |
Result: |
Showed an arrest at the G2/M cell-cycle boundary.
|
Apoptosis Analysis
Cell Line: |
MCF7 cells |
Concentration: |
30 nM, 100 nM and 300 nM |
Incubation Time: |
24 hours or 48 hours |
Result: |
25-30% of cells showed the reduced DNA content characteristic of apoptotic cells.
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