BI8626 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC50 of 0.9 μM.
IC50&Target
IC50: 0.9 μM (HUWE1)
体外研究
BI8626 induces HUWE1 ectopically expresses to abolishe ubiquitination of MCL1 in HeLa cells.
BI8626 suppresses colony formation of Ls174T cells with estimated IC50 value of 0.7 μM, and BI8622 (1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.
BI8626 (0-50 μM; 0-6 hours) retards the degradation of MCL1 in response to UV irradiation to the same extent as depletion of HUWE1 in U2OS cells.
Cell Cycle Analysis
Cell Line:
Ls174T cells
Concentration:
0 μM, 5 μM,10 μM, 15 μM, 20 μM
Incubation Time:
0-4 days
Result:
Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.
Western Blot Analysis
Cell Line:
U2OS cells
Concentration:
0 μM, 20 μM, 50 μM
Incubation Time:
0 hour,1 hour,2 hours,4 hours,6 hours
Result:
Retarded the degradation of MCL1 in response to UV irradiation in HeLa cells by inhibiting HUWE1 in U2OS cells.
分子式
C25H28N8
分子量
440.54
CAS号
1875036-75-1
运输条件
Room temperature in continental US; may vary elsewhere.