NF-κB-IN-1 - CAS:1227098-15-8

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NF-κB-IN-1

目录号 : KM6722 CAS No. : 1227098-15-8 纯度 : 98%

Data Sheet 产品使用指南

NF-κB-IN-1，一种 4-亚芳基姜黄素类似物，是有效的 NF-κB 信号通路抑制剂。NF-κB-IN-1 直接抑制 IKK 来阻断 NF-κB 的激活。NF-κB-IN-1 可有效抑制肺癌细胞的活力并减弱 A549 细胞的克隆活性。

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5mg		In-stock
10mg		In-stock
25mg	询价	In-stock
50mg	询价	In-stock
100mg	询价	In-stock

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生物活性

NF-κB-IN-1, a 4-arylidene crucumin analogue, is a potent NF-κB signaling pathway inhibitor. NF-κB-IN-1 directly inhibits IKK to block NF-κB activation. NF-κB-IN-1 effectively inhibits the viability of lung cancer cells and attenuates the clonogenic activity of A549 cells.

体外研究

NF-κB-IN-1 (compound 17) (0.001-100 μM; 72 h) inhibits the growth of A549, H1944, H460 and H157 cells, with GI₅₀s of 0.72, 0.07, 0.13, and 0.16 μM, respectively.
NF-κB-IN-1 (0.5-25 μM; pretreated for 30 min or 4 h) potently blocks the IκB phosphorylation and degradation in A549 cells.
NF-κB-IN-1 (0.1-100 μM; pretreated for 30 min) dose-dependently inhibits the TNFα-induced nuclear translocation of NF-κB in A549 cells, with an IC₅₀s of 1.0 μM.
NF-κB-IN-1 (0.1-0.4 μM; 9 d) inhibits lung cancer clonogenic activity.

Cell Viability Assay

Cell Line:	A549, H1944, H460 and H157 cells
Concentration:	0.001, 0.01, 0.1, 1, 10, 100 μM
Incubation Time:	72 hours
Result:	Decreased viability of lung adenocarcinoma cells A549 and H1944, quamous cell carcinoma cells H157, and large cell carcinoma cells H460 in a dose-dependent manner.

Western Blot Analysis

Cell Line:	A549 cells
Concentration:	0.5, 1.0, 2.5, 5.0, 10.0, 25.0 μM
Incubation Time:	Pretreated for 30 min or 4 h
Result:	Blocked IκB phosphorylation, with an IC₅₀ of 2.8 μM. Blocked IκB degradation in a dose-dependent manner.

分子式

C₃₁H₃₀O₈

分子量

530.57

CAS号

1227098-15-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 50 mg/mL (94.24 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.8848 mL	9.4238 mL	18.8477 mL
5 mM	0.3770 mL	1.8848 mL	3.7695 mL
10 mM	0.1885 mL	0.9424 mL	1.8848 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (4.71 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (4.71 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。