Sulfo-NHS-SS-biotin is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin can be used for the labeling and purifying of cell-surface protein.
IC50&Target
IC50: cell-surface protein
体外研究
Sulfo-NHS-SS-biotin is a cell-surface-labeling reagent, it is a negatively charged reagent and does not permeate cell membranes.
Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides.
Sulfo-NHS-SS-biotin has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns.
Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail (K0010). Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer.
分子式
C19H27N4NaO9S4
分子量
606.69
CAS号
325143-98-4
运输条件
Room temperature in continental US; may vary elsewhere.