SLM6031434 (hydrochloride) - CAS:1897379-34-8

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SLM6031434 (hydrochloride)

目录号 : KCM12238 CAS No. : 1897379-34-8 纯度 : ≥98%

Data Sheet 产品使用指南

SLM6031434 is an inhibitor of sphingosine kinase 2 (SPHK2) (K = 0.4 μM for the recombinant mouse enzyme). It is selective for SPHK2 over SPHK1 (Ks = >20 μM). It decreases sphingosine-1-phosphate (S1P; ) and increases sphingosine levels in U937 monocytic leukemia cells in a concentration-dependent manner. SLM6031434 (5 mg/kg) reduces blood S1P levels in Sphk1, but not Sphk2, mice. SLM6031434 also increases blood S1P levels in wild-type mice and rats.

规格	价格	是否有货
1mg		In-stock
5mg		In-stock
10mg		In-stock
50mg	询价	In-stock

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Other Forms of Rapamycin:

WAY-272077 In-stock
SLM6031434 (hydrochloride) In-stock
SLM6031434 In-stock
Phorbol 12-myristate 13-acetate In-stock

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生物活性

SLM6031434 (hydrochloride) is the hydrochloride salt form of SLM6031434. SLM6031434 is a highly selective sphingosine kinase 2 (SphK2) inhibitor with an IC50 value of 0.4 μM for SphK2. SLM6031434 exerts anti-fibrotic effects by increasing sphingosine accumulation and Smad7 expression. SLM6031434 demonstrates effective anti-fibrotic efficacy in a unilateral ureteral obstruction (UUO)-induced tubulointerstitial fibrosis mouse model. SLM6031434 can be used for the study of proteinuric kidney diseases or chronic kidney disease (CKD)

IC₅₀&Target

SphK2 SphK1

0.4 μM (IC₅₀) 16 μM (IC₅₀)

体外研究

SLM6031434 (3 μM, 16 h) reduces the expression of TGFβ-induced profibrotic markers (Col1, FN-1, CTGF) in primary mouse renal fibroblasts.
SLM6031434 (0.3-10 μM, 16 h) dose-dependently increased Smad7 protein expression in primary mouse renal fibroblasts.
SLM6031434 (1 μM, 20 h) significantly increased the ceramide levels in human podocytes .
SLM6031434 (1 μM, 24 h) upregulates the protein and mRNA expression of nephrin and Wilms tumor suppressor gene 1 (WT1) in wild-type human podocytes.

体内研究

SLM6031434 (5 mg/kg, intraperitoneal injection, daily, 9 days) alleviates renal interstitial fibrosis induced by unilateral ureteral obstruction in mice.

Animal Model:	UUO-induced renal interstitial fibrosis mouse models were established by performing unilateral ureteral ligation surgery on 10-12 week-old adult male mice
Dosage:	5 mg/kg
Administration:	i.p., daily, 9 days
Result:	Reduced collagen accumulation and ECM deposition. Decreased α-SMA expression to reduce myofibroblast activation. Downregulated mRNA and protein levels of Col1, FN-1, CTGF. Increased sphingosine accumulation and Smad7 expression, while reduced Smad2 phosphorylation. Reduced F4/80-positive macrophage infiltration and downregulated iNOS, Mcr1 mRNA.

分子式

C₂₂H₃₀F₃N₅O₂^.HCl

分子量

489.96

CAS号

1897379-34-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

DMSO: 5 mg/mL (10.20 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.0410 mL	10.2049 mL	20.4098 mL
5 mM	0.4082 mL	2.0410 mL	4.0820 mL
10 mM	0.2041 mL	1.0205 mL	2.0410 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

纯度： ≥98%

Data Sheet

产品使用指南

Schwalm S, et al. Validation of highly selective sphingosine kinase 2 inhibitors SLM6031434 and HWG-35D as effective anti-fibrotic treatment options in a mouse model of tubulointerstitial fibrosis. Cell Signal. 2021 Mar;79:109881.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

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动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

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第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

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计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

Other Forms of Rapamycin:

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器