BIX01294 (hydrochloride hydrate) - CAS:1808255-64-2

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BIX01294 (hydrochloride hydrate)

目录号 : KCM12088 CAS No. : 1808255-64-2 纯度 : ≥98%

Data Sheet 产品使用指南

The methylation of lysine residues on histones plays a central role in determining euchromatin structure and gene expression. The histone methyltransferase (HMTase) G9a can mono- or dimethylate lysine 9 on histone 3 (H3), contributing to early embryogenesis, genomic imprinting, and lymphocyte development. BIX01294 (hydrochloride hydrate) is a selective inhibitor of G9a HMTase (IC = 1.7 μM). It less effectively inhibits the HMTase G9a-like protein (GLP; IC = 38 μM) and has no effect on other known HMTases. BIX01294 has been used in combination with the calcium channel activator BayK8644 to facilitate the generation of induced pluripotent stem cells from somatic cells in vitro.

规格	价格	是否有货	数量
1mg		In-stock
5mg		In-stock
10mg		In-stock
50mg		In-stock

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生物活性

BIX01294 (hydrochloride hydrate) is a histone-lysine methyltransferase (HMTase) inhibitor, which selective inhibits the G9aHMTase with IC₅₀ of 1.7 μM, reduces histone-3 lysine (9) methylation (H3K9me), induces autophagy and apoptosis in human glioma cells.

体外研究

BIX01294 (hydrochloride hydrate) (1-10 μM) induces autophagy and apoptosis in LN18 glioma cells, reducing cell viability. BIX01294 (hydrochloride hydrate) (1-10 μM) upregulates the levels of autophagy-related genes LC3B and WIPI1 while downregulating differentiation-related genes GFAP and TUBB3, promoting autophagy-dependent differentiation in glioma stem-like cells (GSCs) .

Cell Viability Assay

Cell Line:

LN18

Concentration:

1–10 μM

Incubation Time:

24-72 hours

Result:

Reduced cell viability of LN18 in a dose-dependent manner.

Western Blot Analysis

Cell Line:	LN18
Concentration:	1–10 μM
Incubation Time:	24-72 hours
Result:	Increased levels of cleaved caspase3/7 and cleaved PARP. Decreased levels of H3K9me2, H3K27me3 and accumulation of LC3-II.

分子式

C₂₈H₃₈N₆O₂^.₃HCl[XH₂O]

CAS号

1808255-64-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

纯度： ≥98%

Data Sheet

产品使用指南

暂无相关参考文献

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

Other Forms of Rapamycin:

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器