Sodium Selenite - CAS:10102-18-8

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Sodium Selenite (Synonyms:亚硒酸钠)

目录号 : KM32688 CAS No. : 10102-18-8 纯度 : 99%

Data Sheet 产品使用指南

Sodium Selenite 是一种具有口服活性的抗炎、抗氧化和抗肿瘤剂。Sodium Selenite 通过下调TLR4/NF-κB 和 IDO1/kynurenine 通路，显著减轻炎症细胞浸润、氧化应激和细胞凋亡 (apoptosis)，发挥抗结肠炎活性。Sodium Selenite 通过增加 ROS，抑制 NF-κB 信号通路，从而诱导凋亡、抑制 EMT 和血管生成相关蛋白 (MMP-9, VEGF) 发挥抗癌活性。Sodium Selenite 还可缓解淋巴水肿。

规格	价格	是否有货	数量
25mg		In-stock
50mg		In-stock
100mg		In-stock

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Other Forms of Rapamycin:

Sodium Selenite In-stock
4"-Isovalerylspiramycin I In-stock
Higenamine-d4 hydrochloride In-stock
Adebrelimab In-stock
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生物活性

Sodium Selenite is an orally active anti-inflammatory, antioxidant and antitumor agent. Sodium Selenite exerts anti-colitis activity by downregulating the TLR4/NF-κB and IDO1/kynurenine pathways, which significantly reduces inflammatory cell infiltration, oxidative stress and apoptosis. Sodium Selenite exerts anticancer activity by inducing apoptosis, inhibiting EMT and angiogenesis-related proteins (MMP-9, VEGF) via increasing ROS and suppressing the NF-κB signaling pathway. Sodium Selenite also alleviates lymphedema.

体外研究

Sodium selenite (2.5-40 μM; 6-24 h) inhibits the activity of 786-O and ACHN in human renal cancer cells in a time-dependent and dose-dependent manner; After 24 hours of treatment, its IC50 for 786-O cells was 5.15 μM, and its IC50 for ACHN cells was 17.27 μM.
Sodium selenite (5-10 μM; 2 weeks) can dose dependently inhibit the colony formation of human renal cancer cells 786-O and ACHN, with a more significant effect on 786-O cells.
Sodium selenite (5-20 μM; 24 h) inhibits the migration and invasion of human renal cancer cells 786-O and ACHN in a dose-dependent manner.
Sodium selenite (2.5-40 μM; 6 h) can upregulate E-cadherin and downregulate MMP-9 and VEGF at the mRNA and protein levels in a dose-dependent manner in human renal cancer cells 786-O and ACHN.
Sodium selenite (2.5-40 μM; 6 h) can upregulate pro apoptotic proteins Bax and cleaved caspase-3 at the mRNA and protein levels in human renal cancer cells 786-O and ACHN in a dose-dependent manner, while downregulating anti apoptotic proteins Bcl-2, cIAP, and xIAP.
Sodium selenite (5-20 μM; 6 h) can be detected by Annexin V-FITC/PI staining and induces apoptosis in human renal cancer cells 786-O and ACHN in a dose-dependent manner.
Sodium selenite (5-20 μM; 6 h) showed a dose-dependent increase in intracellular ROS levels of 786-O and ACHN in human renal cancer cells, and decreased their mitochondrial membrane potential.
Sodium selenite (2.5-40 μM; 6 h) can dose dependently inhibit the NF - κ B signaling pathway in human renal cancer cells 786-O and ACHN by reducing the levels of p-p65 and p-I κ B - α proteins, blocking the nuclear translocation of p-p65.

Sodium selenite (2 μM; 6 days) reversibly inhibits the differentiation of human CD4+T cells into CD4+CD25+Foxp3+and CD4+CTLA-4+regulatory T cells; Under the condition of no regulatory T cell differentiation stimulation, pretreatment of CD4+T cells with sodium selenite can increase the proportion of CD4+CTLA-4+cells .
Sodium selenite (2 μM; 6 days) can regulate the mRNA expression of TET2, TET3, and IL-10 in human CD4+T cells, reduce TET3 expression during Treg differentiation, and upregulate IL-10 expression in undifferentiated CD4+T cell culture system.
Sodium selenite (2 μM; 6 days) can increase the level of IL-10 protein in human CD4+T cell cultures stimulated only by CD3/CD28, with no significant difference compared to the untreated group stimulated only by CD3/CD28.
Sodium selenite can inhibit the proliferation of HL-60 cells, induce apoptosis in human promyelocytes, lymphoma, and glioblastoma cells, and exert cytotoxic effects in human primary AML cells.

Cell Viability Assay

Cell Line:	human renal cancer 786-O cells, human renal cancer ACHN cells
Concentration:	2.5, 5, 10, 20 and 40 μM
Incubation Time:	6; 12; 24 hours
Result:	Reduced cell viability in a time- and dose-dependent manner in both cell lines.

Cell Invasion Assay

Cell Line:	human renal cancer 786-O cells, human renal cancer ACHN cells
Concentration:	5, 10 and 20 μM
Incubation Time:	24 hours
Result:	Dose-dependently inhibited wound closure in both cell lines.

Apoptosis Analysis

Cell Line:	human renal cancer 786-O cells, human renal cancer ACHN cells
Concentration:	5, 10 and 20 μM
Incubation Time:	6 hours
Result:	Dose-dependently increased total apoptotic cell percentage (early + late apoptosis) in both cell lines.

体内研究

Sodium selenite (0.5 mg/kg; gavage; Administration once each on day 0 and day 1 after induction can alleviate acetic acid-induced colitis in rats by reducing inflammatory cell infiltration, oxidative stress, pro-inflammatory cytokine production, and activation of inflammatory signaling pathways, while increasing the anti apoptotic Bcl-2/Bax ratio.
Sodium selenite (2 mg/kg; intraperitoneal injection; Once every 2 days; A total of 14 days can significantly inhibit the growth of renal cell carcinoma xenografts, induce tumor cell apoptosis, and reduce the expression of phosphorylated NF - κ B p65 in BALB/c nude mice.
Sodium Selenium (dietary supplement) can enhance humoral immunity by increasing the titers of anti SRBC IgG and IgM, and can also enhance cellular immunity in mice by increasing target cell lysis levels, NK mediated cytotoxicity, and CTL activity.
Sodium Selenium (low high dose; Dietary supplementation can shift the differentiation balance of CD4+T cells in peptide stimulated mice towards Th1 phenotype, and a high selenium diet can increase the levels of interferon - γ, CD40L, and T cell signaling markers.
Sodium Selenium (low high dose; Research on dietary supplementation has shown that moderate dose dietary supplementation can promote Th2 polarization in OVA sensitized mice, which can be confirmed by elevated levels of TNF - α, IL-4, IL-5 in the airways and pSTAT-6 in lung tissue.

Animal Model:	Wistar rats (male, 10 to 12 weeks old, 200 to 250 g, acetic acid-induced colitis)
Dosage:	0.5 mg/kg
Administration:	oral gavage; twice (day 0 and day 1 post-induction)
Result:	Alleviated colonic injury signs including edema, granulation, erythema, and necrosis, with more perceptible improvements in lower acetic acid concentration groups. Reduced submucosal inflammatory cell counts, mitigated cryptitis, crypt abscess formation, and mucosal/submucosal tissue architecture derangement. Markedly reduced tissue malondialdehyde (MDA) levels and significantly decreased myeloperoxidase (MPO) activity.

Animal Model:	BALB/c athymic (6-8 weeks of age)
Dosage:	2 mg/kg
Administration:	i.p.; every other day; 14 days
Result:	Significantly reduced final tumor volume relative to controls. Significantly decreased final tumor weight relative to controls. Showed no significant difference in body weight compared to controls. Increased the percentage of apoptotic (TUNEL-positive) cells in tumors significantly. Increased tumor tissue expression of E-cadherin significantly. Increased tumor tissue expression of cleaved caspase-3 significantly. Decreased tumor tissue expression of VEGF significantly. Decreased tumor tissue expression of phosphorylated NF-κB p65 significantly.

分子式

Na₂O₃Se

分子量

172.94

CAS号

10102-18-8

中文名称

亚硒酸钠

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H2O: ≥ 200 mg/mL (1156.47 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	5.7824 mL	28.9118 mL	57.8235 mL
5 mM	1.1565 mL	5.7824 mL	11.5647 mL
10 mM	0.5782 mL	2.8912 mL	5.7824 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

纯度： 99%

Data Sheet

产品使用指南

Liu X, et al. Sodium selenite inhibits proliferation and metastasis through ROS-mediated NF-κB signaling in renal cell carcinoma. BMC Cancer. 2022;22(1):870. Published 2022 Aug 9.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

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动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

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第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

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计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

Other Forms of Rapamycin:

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器