N-3-oxo-dodecanoyl-L-Homoserine lactone - CAS:168982-69-2

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N-3-oxo-dodecanoyl-L-Homoserine lactone (Synonyms:N-(3-氧代十二酰基)-L-高丝氨酸内酯;N-(3-Oxododecanoyl)-L-homoserine lactone;OdDHL)

目录号 : KCM11925 CAS No. : 168982-69-2 纯度 : ≥98%

Data Sheet 产品使用指南

N-3-oxo-dodecanoyl-L-Homoserine lactone (3-oxo-C12-HSL) is a bacterial quorum-sensing signaling molecule produced by P. aeruginosa and strains of the B. cepacia complex. It induces the production of IL-8 in 16HBE human bronchial epithelial cells when used at a concentration of 100 µM. Supernatants from 3-oxo-C12-HSL-stimulated 16HBE cells induce chemotaxis of isolated human neutrophils. 3-oxo-C12-HSL has been found in respiratory secretions from patients with cystic fibrosis infected with P. aeruginosa.

规格	价格	是否有货	数量
10 mM * 1 mL in DMSO		In-stock
5mg		In-stock
10mg		In-stock
50mg		In-stock

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生物活性

N-3-oxo-dodecanoyl-L-Homoserine lactone is a bacterial quorum-sensing signaling molecule produced by P. aeruginosa and strains of the B. cepacia complex.Quorum sensing is a regulatory system used by bacteria for controlling gene expression in response to increasing cell density.N-3-oxo-dodecanoyl-L-Homoserine lactone induces the production of IL-8 in 16HBE human bronchial epithelial cells.

体外研究

N-3-oxo-dodecanyl-L-homoserine lactone (25-100 μM, 18 h) inhibits IL-8 expression and induces neutrophil chemotaxis in L828 cells by activating NF - κ B and ERK1/2.
N-3-oxo-dodecanyl-L-homoserine lactone (50 μM, 24 h) induces ER stress response, ROS generation, and mitochondrial membrane potential reduction, leading to caspase 3/8/9/12 activation and inducing apoptosis in BMDM.

Apoptosis Analysis

Cell Line:	BMDM
Concentration:	0-50 μM
Incubation Time:	24 hours
Result:	Induced apoptosis in BMDMs.

Western Blot Analysis

Cell Line:	BMDM
Concentration:	0-50 μM
Incubation Time:	1 hour
Result:	Increased cleaved caspase 3/8/9/12.

体内研究

N-3-oxo-dodecanoyl-L-homoserine lactone (0.3-1 mg/kg, ip, single dose) enhanced the immune preference of the host, showed immunomodulatory activity in OVA immunized mice model, and improved type 1 diabetes by inhibiting the activation and differentiation of initial T cells.

Animal Model:	OVA-immunized mouse models
Dosage:	0.3 mg/kg
Administration:	ip, single dose
Result:	Increased OVA-specific IgG1 (Th2-related antibody).

分子式

C₁₆H₂₇NO₄

分子量

297.39

CAS号

168982-69-2

中文名称

N-3-氧代十二酰基-L-高丝氨酸内酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 100 mg/mL (336.26 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.3626 mL	16.8129 mL	33.6259 mL
5 mM	0.6725 mL	3.3626 mL	6.7252 mL
10 mM	0.3363 mL	1.6813 mL	3.3626 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO → 40% PEG300 → 5% Tween-80 → 45% saline

Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (8.41 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO → 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (8.41 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
3.

请依序添加每种溶剂： 10% DMSO → 90% corn oil

Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (8.41 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

纯度： ≥98%

Data Sheet

产品使用指南

Chambers CE, Visser MB, Schwab U, Sokol PA. Identification of N-acylhomoserine lactones in mucopurulent respiratory secretions from cystic fibrosis patients. FEMS Microbiol Lett. 2005 Mar 15;244(2):297-304.

Smith RS, Fedyk ER, Springer TA, Mukaida N, Iglewski BH, Phipps RP. IL-8 production in human lung fibroblasts and epithelial cells activated by the Pseudomonas autoinducer N-3-oxododecanoyl homoserine lactone is transcriptionally regulated by NF-kappa B a

Ritchie AJ, et al., Modification of in vivo and in vitro T- and B-cell-mediated immune responses by the Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)-L-homoserine lactone. Infect Immun. 2003 Aug;71(8):4421-31.

Gaisford W, et al., OdDHL inhibits T cell subset differentiation and delays diabetes onset in NOD mice. Clin Vaccine Immunol. 2011 Aug;18(8):1213-20.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

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动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器