(S)-4'-nitro-Blebbistatin - CAS:1621326-32-6

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(S)-4'-nitro-Blebbistatin (Synonyms:p-硝基布雷他汀;para-Nitroblebbistatin;p-Nitroblebbistatin)

目录号 : KCM11806 CAS No. : 1621326-32-6 纯度 : ≥95%

Data Sheet 产品使用指南

(S)-4'-nitro-Blebbistatin is a more stable and less phototoxic form of (–)-blebbistatin , which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases. (–)-Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (ICs = 0.5-5 µM), while poorly inhibiting smooth muscle myosin (IC = 80 µM). Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications. The addition of a 4’-nitro group decreases the inherent fluorescence, stabilizes the molecule to circumvent its degradation by prolonged blue light exposure, and decreases its phototoxicity while retaining the in vitro and in vivo activity of (–)-blebbistatin. (S)-4'-nitro-Blebbistatin has the same stereochemistry as the active (–)-blebbistatin enantiomer.

规格	价格	是否有货	数量
10 mM * 1 mL in DMSO		In-stock
1mg		In-stock
5mg		In-stock
10mg		In-stock

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生物活性

(S)-4'-nitro-Blebbistatin is a derivative of Blebbistatin and an inhibitor of myosin II. (S)-4'-nitro-Blebbistatin is photostable, non-cytotoxic, and non-phototoxic. (S)-4'-nitro-Blebbistatin can serve as an ideal substitute for Blebbistatin to study the role of myosin II in physiology, development, and cell biology[1].

体外研究

(S) -4 '- nitro-Blebistatin (0.31-10 μM; 24 h) can inhibit cytoplasmic division of COS-7 cells, resulting in a multinucleated phenotype with an EC50 of 2.9 μM [1].

(S) -4 '- nitro-Blebistatin (10 μM; 480 nM) was irradiated for 15 minutes; 18 hours) showed no cytotoxicity in HeLa cells, and there was no difference in cell mortality and morphology compared to the control group.

体内研究

(S) -4 '- nitro-Blebistatin (5-20 μM; single dose; 24 hours) In transgenic cldnb: gfp zebrafish embryos, cell migration of the posterior line primordia can be inhibited, resulting in a curved body shape of zebrafish embryos. This effect is similar to Blebbistatin, but the mortality rate of animals in the (S) -4 '- nitro-Blebistatin group is significantly reduced.

分子式

C₁₈H₁₅N₃O₄

分子量

337.33

CAS号

1621326-32-6

中文名称

p-Nitroblebbistatin

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO: 22 mg/mL (65.22 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9645 mL	14.8223 mL	29.6446 mL
5 mM	0.5929 mL	2.9645 mL	5.9289 mL
10 mM	0.2964 mL	1.4822 mL	2.9645 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

纯度： ≥95%

Data Sheet

产品使用指南

[1]. Radnai L, et al. A simple and robust cell-based assay for the discovery of novel cytokinesis inhibitors. J Biol Methods. 2020 Sep 17;7(3):e136.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

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动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

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% Tween 80

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工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器