(6R,S)-5,6,7,8-Tetrahydrofolic Acid (hydrochloride) - CAS:150731-85-4

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(6R,S)-5,6,7,8-Tetrahydrofolic Acid (hydrochloride) (Synonyms:四氢叶酸三盐酸盐;Tetrahydrofolic acid trihydrochloride;L-Tetrahydrofolic acid trihydrochloride)

目录号 : KCM11585 CAS No. : 150731-85-4 纯度 : ≥95%

Data Sheet 产品使用指南

(6R,S)-5,6,7,8-Tetrahydrofolic acid (THFA), the reduced form of folic acid, serves as a cofactor in methyltransferase reactions and is the major one-carbon carrier in one carbon metabolism. Its metabolites participate in the synthesis of thymidine for incorporation into DNA or the synthesis of purines, as well as in the formation of methionine, which can be converted to the ubiquitous methyl donor, S-adenosylmethionine . THFA has also been used to study the activation of metabolite-binding riboswitches.

规格	价格	是否有货	数量
1mg		In-stock
5mg		In-stock
10mg		In-stock
25mg		In-stock

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生物活性

(6R,S)-5,6,7,8-Tetrahydrofolic Acid (hydrochloride) is the biologically active vitamin B9 folate derivative. (6R,S)-5,6,7,8-Tetrahydrofolic Acid (hydrochloride) is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. (6R,S)-5,6,7,8-Tetrahydrofolic Acid (hydrochloride) serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

体外研究

(6R, S) -5,6,7,8-Tetrahydrofolic Acid (hydrochloride) (0-200 μM; 3 days; Exposure to trihydrochloride in Adh5-/- DT40 cells exhibits cytotoxicity towards Adh5 and Fanconi anemia (FA) deficient cells, resulting in extensive DNA damage and chromosome accumulation interruption.
(6R, S) -5,6,7,8-Tetrahydrofolic Acid (hydrochloride) (0-100 μM; 16 hours; Treatment with trihydrochloride in Adh5-/- DT40 cells strongly promotes the dose-dependent formation of FANCD2 and ser139-H2AX focusing in Adh5-/- cells.
Due to uncontrolled activity, exposure to (6R, S) -5,6,7,8-Tetrahydrofolic Acid (hydrochloride) activates the action of DNA damage response (DDR) thymidylate synthase, leading to depletion of essential nucleotides and promoting repair through homologous recombination mechanisms.

Cell Viability Assay

Cell Line:	Adh5^-/- DT40 cells
Concentration:	0-200 µM
Incubation Time:	3 days
Result:	Viability of Adh5^-/-DT40 cells rapidly dropped.

Western Blot Analysis

Cell Line:	Adh5^-/- DT40 cells
Concentration:	0-200 µM
Incubation Time:	16 hours
Result:	Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5^-/- cells in a dose-dependent manner.

体内研究

(6R, S) -5,6,7,8-Tetrahydrofolic Acid (hydrochloride) (62.5 mg/kg; intraperitoneal injection; Every day; The treatment of Adh5-/- mice disrupts the hematopoietic function of hematopoietic cells, increases phosphorylation of ser139-H2AX, and reduces the survival rate of progenitor cells (HSPCs), indicating that excessive Tetrahydrofolic acid trihydrochloride may have mutagenic and genotoxic effects on bone marrow cells.

Animal Model:	Adh5^-/- mice
Dosage:	62.5 mg/kg
Administration:	Intraperitoneal injection; daily
Result:	Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).

分子式

C₁₉H₂₃N₇O₆^.₃HCl

分子量

554.81

CAS号

150731-85-4

中文名称

四氢叶酸三盐酸盐;

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

纯度： ≥95%

Data Sheet

产品使用指南

Steven W. Bailey, et al. Synthesis of Tetrahydropteridine C6-Stereoisomers, Including N6-Formyl-(68)-tetrahydrofolic Acid. The Journal of Organic Chemistry, 57(16), 4470-4477.

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Other Forms of Rapamycin:

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