BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC50 of 3.1 μM.
IC50&Target
IC50: 3.1 μM (HUWE1)
体外研究
BI8622 induces HUWE1 ectopically expresses to abolish ubiquitination of MCL1 with an IC50 value of 6.8 μM in HeLa cells.
BI8622 suppresses colony formation of Ls174T cells with estimated IC50 value of 8.4 μM.
BI8622 (10 μM; 1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.
BI8622 (0-50 μM; 16 hours) retards the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells.
BI8622 inhibits MYC-dependent transactivation in colorectal cancer cells.
Cell Cycle Analysis
Cell Line:
Ls174T cells
Concentration:
0 μM, 5 μM,10 μM, 15 μM, 20 μM
Incubation Time:
0-4 days
Result:
Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.
Western Blot Analysis
Cell Line:
HeLa cells
Concentration:
0 μM, 10 μM, 20 μM
Incubation Time:
16 hours
Result:
Retarded the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells.
分子式
C25H26N6O
分子量
426.51
CAS号
1875036-74-0
运输条件
Room temperature in continental US; may vary elsewhere.