2,5-Dihydroxycinnamic Acid phenethyl ester - CAS:179691-97-5

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2,5-Dihydroxycinnamic Acid phenethyl ester (Synonyms:5-LOX-IN-2)

目录号 : KCM12062 CAS No. : 179691-97-5 纯度 : ≥98%

Data Sheet 产品使用指南

2,5-Dihydroxycinnamic acid phenethyl ester is an inhibitor of 5-lipoxygenase (5-LO) with an IC value of 0.33 µM for inhibition of 5-LO product synthesis in polymorphonuclear leukocytes (PMNLs) stimulated with the sarcoendoplasmic reticulum calcium ATPase (SERCA) inhibitor thapsigargin . It scavenges 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (IC = 16.41 µM) in a cell-free assay. 2,5-Dihydroxycinnamic acid phenethyl ester selectively decreases viability of RCC4 and 786-O cells lacking the Von Hippel-Lindau (VHL) tumor suppressor gene (ICs = 8 and 34.8 µM, respectively) over cells homozygously expressing VHL (ICs = 99.6 and >100 µM, respectively). However, it also decreases viability of RCC10 VHL and RCC10 VHL cells (ICs = 5.04 and 0.96 µM, respectively). It increases the levels of pro-caspase-3a, LC3B-I, and LC3B-II in RCC4 VHL cells in a concentration-dependent manner.

规格	价格	是否有货	数量
10 mM * 1 mL in DMSO		In-stock
1mg		In-stock
5mg		In-stock
10mg		In-stock
25mg		In-stock

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生物活性

2,5-Dihydroxycinnamic Acid phenethyl ester , an inhibitor of 5-lipoxygenase (5-LOX) with an IC50 of 0.33 μM, inhibits 5-LOX in a dose-dependent manner . 2,5-Dihydroxycinnamic Acid phenethyl ester, reduces the cell viability of renal cancer cells and induces apoptosis, can be used for cancer research.

IC₅₀&Target

5-LOX

0.33 μM (IC50)

体外研究

2,5-dihydroxycinnamic Acid phenyl ester (0-100 μM; 4 days) can reduce the viability of renal cancer cells and is more selective towards RCC4 and 786.0 cells lacking the Von Hippel Lindau (VHL) tumor suppressor gene.
2,5-dihydroxycinnamic Acid phenyl ester (0-10 μM; 24 hours) can increase the expression of LC3B and p62, block autophagy flux in RCC4 cells, and induce apoptosis mediated cell death by activating the caspase-3 pathway.

Cell Viability Assay

Cell Line:

RCC4, RCC10, 786.0 cells

Concentration:

0-100 μM

Incubation Time:

4 days

Result:

Reduced the cell viability of renal cancer cells and was more selective toward RCC4 and 786.0 cells which are deficient for the Von Hippel-Lindau (VHL) tumor suppressor gene.

Western Blot Analysis

Cell Line:	RCC4, RCC10, 786.0 cells
Concentration:	0-10 μM
Incubation Time:	24 hours
Result:	Increased in LC3B and p62 expression, blocked of the autophagic flux in RCC4 cells. Stimulated in a dose ependent manner the cleavage of pro-caspase-3 only in the RCC4 cells which lack the VHL tumor suppressor.

分子式

C₁₇H₁₆O₄

分子量

284.31

CAS号

179691-97-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 100 mg/mL (351.73 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.5173 mL	17.5864 mL	35.1729 mL
5 mM	0.7035 mL	3.5173 mL	7.0346 mL
10 mM	0.3517 mL	1.7586 mL	3.5173 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂：10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (8.79 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

纯度： ≥98%

Data Sheet

产品使用指南

Selka A, et.al. Discovery of a novel 2,5-dihydroxycinnamic acid-based 5-lipoxygenase inhibitor that induces apoptosis and may impair autophagic flux in RCC4 renal cancer cells. Eur J Med Chem. 2019 Oct 1;179:347-357.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器