Pyronin Y - CAS:92-32-0

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Pyronin Y (Synonyms:派洛宁Y; Pyronine G; C.I. 45005)

目录号 : KM17165 CAS No. : 92-32-0

Data Sheet 产品使用指南

Pyronin Y (Pyronine G) 是一种可插入 RNA 的阳离子染料，可以靶向包括 RNA，DNA 和细胞器的细胞结构。Pyronin Y 与双链核酸(尤其是 RNA) 形成荧光复合物，可以对细胞 RNA 进行半定量分析。Pyronin Y 可用于鉴定活细胞中核糖核蛋白复合物的特定 RNA 亚种。

规格	价格	是否有货
100mg		In-stock
200mg	询价	In-stock
500mg	询价	In-stock

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Cy5 maleimide In-stock
2-Aminopurine In-stock
7-Aminoactinomycin D In-stock
Pyronin Y In-stock
DAPI dihydrochloride In-stock

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生物活性

Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells.

体外研究

Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin.
A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting.
In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells.
Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent.
Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA).

分子式

C₁₇H₁₉ClN₂O

分子量

302.80

CAS号

92-32-0

中文名称

派洛宁Y；吡罗红G

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 25 mg/mL (82.56 mM; Need ultrasonic)

H₂O : 4 mg/mL (13.21 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.3025 mL	16.5126 mL	33.0251 mL
5 mM	0.6605 mL	3.3025 mL	6.6050 mL
10 mM	0.3303 mL	1.6513 mL	3.3025 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

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