Psoralidin - CAS:18642-23-4

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Psoralidin (Synonyms:补骨脂定)

目录号 : KM16363 CAS No. : 18642-23-4 纯度 : 98%

Data Sheet 产品使用指南

Psoralidin 是 COX-2 和 5-LOX 的双重抑制剂。具有抗癌，抗菌和抗炎作用。Psoralidin 显著下调 NOTCH1 信号传导。Psoralidin 还极大地诱导 ROS 产生。

规格	价格	是否有货
5mg		In-stock
10mg		In-stock
50mg	询价	In-stock
100mg	询价	In-stock

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生物活性

Psoralidin is a dual inhibitor of COX-2 and 5-LOX, regulates ionizing radiation (IR)-induced pulmonary inflammation.Anti-cancer, anti-bacterial, and anti-inflammatory properties. Psoralidin significantly downregulates NOTCH1 signaling. Psoralidin also greatly induces ROS generation.

体外研究

Three breast cancer cell (BCC)populations (ALDH cells, ALDH cells, and commercial BSCSs) are sensitive to Psoralidin treatment (10, 15, 20, and 25 μM; 24 hours) with IC₅₀s ranging from 18 to 21 μM; however, the MCF-12A cells were resistant to Psoralidin.
Psoralidin (30 μM; 24 hours) results in a significant induction of apoptosis for ALDH cells, ALDH cells, and commercial BCSCs.
Psoralidin treatment also downregulates NOTCH1 expression in both ALDH and ALDH cells.

Cell Viability Assay

Cell Line:	ALDH cells, ALDH cells, commercial breast cancer stem cells (BSCSs), and normal breast epithelial cells (MCF-12A)
Concentration:	10, 15, 50, and 25 μM
Incubation Time:	24 hours
Result:	IC₅₀s of 18 to 21 μM for ALDH cells, ALDH cells, commercial BCSCs.

Apoptosis Analysis

Cell Line:	ALDH cells, ALDH cells, and commercial BCSCs
Concentration:	20 and 30 μM
Incubation Time:	24 hours
Result:	No significant induction of apoptosis was observed for any of the three cell types following treatment at 20 μM. However, 53.60%, 44.1%, and 45.9% of cells were apoptotic at 30 μMin ALDH cells, ALDH cells, and commercial BCSCs, respectively.

体内研究

Psoralidin (5 mg/kg) regulates expression of pro-inflammatory cytokines that play an important role in inflammatory diseasesin IR-irradiated lung of BALB/c mouse.

Animal Model:	BALB/c mice
Dosage:	5 mg/kg
Administration:	Intraperitoneal application; 30 min before and 1 h after IR irradiation (20 Gy).
Result:	Anti-inflammatory effect in IR-irradiated mice.

分子式

C₂₀H₁₆O₅

分子量

336.34

CAS号

18642-23-4

中文名称

补骨脂定；补骨脂次素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 50 mg/mL (148.66 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9732 mL	14.8659 mL	29.7318 mL
5 mM	0.5946 mL	2.9732 mL	5.9464 mL
10 mM	0.2973 mL	1.4866 mL	2.9732 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2.08 mg/mL (6.18 mM); Suspended solution; Need ultrasonic

此方案可获得 2.08 mg/mL (6.18 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (6.18 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.18 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。