Psoralidin is a dual inhibitor of COX-2 and 5-LOX, regulates ionizing radiation (IR)-induced pulmonary inflammation.Anti-cancer, anti-bacterial, and anti-inflammatory properties. Psoralidin significantly downregulates NOTCH1 signaling. Psoralidin also greatly induces ROS generation.
IC50&Target
COX-2
5-LOX
体外研究
Three breast cancer cell (BCC)populations (ALDH cells, ALDH cells, and commercial BSCSs) are sensitive to Psoralidin treatment (10, 15, 20, and 25 μM; 24 hours) with IC50s ranging from 18 to 21 μM; however, the MCF-12A cells were resistant to Psoralidin.
Psoralidin (30 μM; 24 hours) results in a significant induction of apoptosis for ALDH cells, ALDH cells, and commercial BCSCs.
Psoralidin treatment also downregulates NOTCH1 expression in both ALDH and ALDH cells.
Cell Viability Assay
Cell Line:
ALDH cells, ALDH cells, commercial breast cancer stem cells (BSCSs), and normal breast epithelial cells (MCF-12A)
Concentration:
10, 15, 50, and 25 μM
Incubation Time:
24 hours
Result:
IC50s of 18 to 21 μM for ALDH cells, ALDH cells, commercial BCSCs.
Apoptosis Analysis
Cell Line:
ALDH cells, ALDH cells, and commercial BCSCs
Concentration:
20 and 30 μM
Incubation Time:
24 hours
Result:
No significant induction of apoptosis was observed for any of the three cell types following treatment at 20 μM. However, 53.60%, 44.1%, and 45.9% of cells were apoptotic at 30 μMin ALDH cells, ALDH cells, and commercial BCSCs, respectively.
体内研究
Psoralidin (5 mg/kg) regulates expression of pro-inflammatory cytokines that play an important role in inflammatory diseasesin IR-irradiated lung of BALB/c mouse.
Animal Model:
BALB/c mice
Dosage:
5 mg/kg
Administration:
Intraperitoneal application; 30 min before and 1 h after IR irradiation (20 Gy).
Result:
Anti-inflammatory effect in IR-irradiated mice.
分子式
C20H16O5
分子量
336.34
CAS号
18642-23-4
中文名称
补骨脂定;补骨脂次素
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
溶解性数据
In Vitro:
DMSO : 50 mg/mL (148.66 mM; ultrasonic and warming and heat to 60°C)