Actinomycin D (Dactinomycin) inhibits DNA repair with an IC50 of 0.42 μM. Actinomycin D is an autophagy activator.
IC50&Target
IC50: 0.42 μM (DNA repair)
体外研究
Actinomycin D (Dactinomycin) is an inhibitor of DNA transcription and replication. Actinomycin D markedly reduces the vascular smooth muscle cells (SMC) proliferation via the inhibition of BrdU incorporation at 80 nM. This is further supported by the G1-phase arrest using a flowcytometric analysis. Actinomycin D is extremely potent with an inhibitory concentration IC50 at 0.4 nM, whereas the lethal dose LD50 is at 260 microM. The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf are all suppressed by Actinomycin D. Extracellular signal-regulated kinases (Erk) involved in cell-cycle arrest are found to increase by Actinomycin D.
体内研究
The pluronic gel containing 80 nM and 80 μM Actinomycin D (Dactinomycin) is applied topically to surround the rat carotid adventitia, the thickness of neointima is substantially reduced (45 and 55%, respectively). Mice in the Actinomycin D and NSC 118218 group lives significantly longer than the control group with P values of <0.001 and 0.007, respectively. Interestingly, single treatment with Actinomycin D is superior to NSC 118218 regarding overall survival (P=0.026).
分子式
C62H86N12O16
分子量
1255.42
CAS号
50-76-0
中文名称
放线菌素 D
运输条件
Room temperature in continental US; may vary elsewhere.