Cyclopamine is a Hedgehog (Hh) pathway antagonist with an IC50 of 46 nM in the Hh cell assay. Cyclopamine is also a selective Smo inhibitor.
体外研究
Treatment with small molecule Hh inhibitors such as HhAntag and the natural product Cyclopamine, both binding to Smo, induces tumor remission in a genetic mouse model of medulloblastoma. Cyclopamine is a Hedgehog (Hh) pathway antagonist. Cyclopamine suppresses cell growth. Cyclopamine (3 μM) suppression of Hh pathway activity and growth in digestive tract tumour cell lines correlates with expression of PTCHmRNA. Cyclopamine is a steroidal alkaloid that inhibits Hh signalling through direct interaction with Smo.
体内研究
Cyclopamine causes durable regression of xenograft tumors. Tumors in Cyclopamine-treated animals, regress completely by 12 days. Cyclopamine (1.2 mg) treatment blocks tumour formation of human pancreatic adenocarcinoma cells after transplantation into nude mice.
分子式
C27H41NO2
分子量
411.62
CAS号
4449-51-8
中文名称
环杷明;环巴胺;11-去氧芥芬胺
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
溶解性数据
In Vitro:
Ethanol : 33.33 mg/mL (80.97 mM; Need ultrasonic)
DMSO : 10 mg/mL (24.29 mM; ultrasonic and warming and heat to 80°C)