Cearoin (10-80 μM) induces cell death in a dose-dependent manner.
Cearoin (10-80 μM) increases the phosporylation of ERK in SH-SY5Y cells.
Cearoin (5-80 μM) increases the conversion of LC3B-I to LC3B-II in SH-SY5Y cells. The expression of LC3B-II is a good marker for autophagosome formation in the autophagy process.
Cell Viability Assay
| Cell Line: |
Human neuroblastoma SH-SY5Y cells |
| Concentration: |
0, 1, 5, 10, 20, 40, or 80 μM |
| Incubation Time: |
6 or 12 hours |
| Result: |
Significantly decreased cell viability from 10 μM in a dose-dependent manner. Treatment with 40 μM for 12 h induced about 50% loss in cell viability in SH-SY5Y cells. |
Western Blot Analysis
| Cell Line: |
Human neuroblastoma SH-SY5Y cells |
| Concentration: |
0, 5, 10, 20, 40, or 80 μM |
| Incubation Time: |
12 hours |
| Result: |
Increased ERK phosphorylation in a dose-dependent manner, whereas it did not alter JNK phosphorylation.
Induced the formation of LC3B-II in a dose dependent manner. |