PMSF is an irreversible serine/cysteine protease inhibitor commonly used in the preparation of cell lysates.
体外研究
PMSF (2 mM) inhibits carbachol-stimulated inositol phosphate accumulation in the presence of Li by only 15%-19%. PMSF inhibition of phosphoinositide turnover is due to one or more steps following phosphoinositide breakdown. PMSF inhibits the acylation of the inositol residue of GPI intermediates in bloodstream form T. brucei. PMSF inhihits the formntion of glycolipid C but does not inhibit fatty acid remodeling in vitro. PMSF inhihits GPI acylation and ethanolamine phosphatp addition in procyclic trypanosomes but not in Hela cells.
体内研究
PMSF (0.1 mL/10 g b.wt, i.p.) produces antinociception as indicated by the dose-responsive increase in % MPE in the tail-flick latency evaluation, but fails to produce a clear dose-responsive inhibition of locomotion. Mice receiving i.p. injections of PMSF exhibit cannabinoid effects that includes antinociception, hypothermia and immobility with ED50 values of 86, 224 and 206 mg/kg, respectively. PMSF (30 mg/kg) pretreatment potentiates the effects of anandamide on tail-flick response (antinociception), spontaneous activity and mobility by 5-, 10- and 8-fold, respectively.
分子式
C7H7O2FS
分子量
174.19
CAS号
329-98-6
中文名称
苯甲基磺酰氟;苄磺酰氟;苄基磺酰氟;苯甲磺酰氟;苯磺基氟化物;苯甲基磺酰氟化物;苯甲基磺酰化氟
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
溶解性数据
In Vitro:
DMSO : 250 mg/mL (1435.21 mM; Need ultrasonic)
Isopropanol : 60 mg/mL (344.45 mM; Need ultrasonic)