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BODIPY FL-C16 is a BODIPY-labeled analog of Palmitic acid, which serves as a fluorescent lipid tracer. BODIPY FL-C16 also acts as a ligand for liver fatty acid-binding protein (L-FABP) and intestinal fatty acid-binding protein (I-FABP) , with Kd values of 270 nM and 330 nM, respectively. BODIPY FL-C16 is rapidly taken up by cells, and after metabolic conversion to phospholipids, it is incorporated into the membrane structures of intracellular organelles and extracellular vesicles[1].

BODIPY FL-C16 (1-7 μM; 15 min-5 h pulse, up to 24 h chase) can be used for metabolic labeling of a homogeneous subpopulation of small extracellular vesicles originating from endosomes/multivesicular bodies (MVBs) with a particle size of approximately 80 nm in human Mel501, Me665/1, A375/M, HEK293T, and HeLa cells; After 2 hours of pulse treatment and 6 hours later, the secretion of vesicles reached the peak of the plateau period, and the labeled vesicles were highly enriched in CD63, moderately enriched in CD81, and weakly enriched in CD9 [1].

The fluorescence quantum yield of BODIPY FL-C16 (0.5 μM; 5 μM L-FABP/I-FABP) in PBS-CGM buffer is relatively low, only 0.03, while the quantum yield in methanol is relatively high, about 0.93; When combined with recombinant rat L-FABP and I-FABP separately, their quantum yields increased to approximately 0.87 and 0.72, respectively [2].

BODIPY FL-C16 (0.5 μM;~1 min) can bind to recombinant rat L-FABP with an apparent KD value of 270 nM; it can also bind to recombinant rat I-FABP with an apparent KD value of 330 nM, occupying the fatty acid binding sites on I-FABP and one of the two fatty acid binding sites on L-FABP [2].

BODIPY FL-C16 (5 μM; 15 min at 37 ° C) is widely co localized with mitochondria, endoplasmic reticulum/Golgi apparatus in fully differentiated human intestinal Caco-2 cells, but not with lysosomes [2].

BODIPY FL-C16 (5 μM; 15 min at 37 ° C) does not undergo esterification or integration into complex lipids in fully differentiated human intestinal Caco-2 cells incubated at 37 ° C for 15 minutes [2].

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)