G-418（Geneticin，遗传霉素）作为一种氨基糖苷类抗生素，是分子遗传学实验中用于稳定转染筛选的主流抗性试剂。其作用机制在于干扰80S核糖体功能，从而抑制真核细胞蛋白质合成，广泛应用于哺乳动物、植物及酵母细胞的选择性培养。该抗生素对细菌、真菌、高等植物、哺乳动物细胞、原虫及蠕虫均具毒性，其显性抗性基因位于Tn601（903）和Tn5转座子上。该细菌来源的基因可在真核细胞中有效表达，赋予宿主G-418抗性，使其能在含药培养基中存活。凭借这一特性，G-418已成为基因转移、基因敲除、抗性筛选及转基因动物研究中的常用工具。
| 生物活性 |
G-418 (Geneticin), as an aminoglycoside antibiotic, is a mainstream resistance reagent used for stable transfection screening in molecular genetics experiments. Its mechanism of action is to interfere with the function of the 80S ribosome, thereby inhibiting protein synthesis in eukaryotic cells. It is widely used in selective culture of mammalian, plant, and yeast cells. This antibiotic is toxic to bacteria, fungi, higher plants, mammalian cells, protozoa, and worms. Its dominant resistance genes are located on Tn601 (903) and Tn5 transposons. The gene derived from this bacterium can be effectively expressed in eukaryotic cells, endowing the host with G-418 resistance and enabling it to survive in drug containing culture medium. With this characteristic, G-418 has become a commonly used tool in gene transfer, gene knockout, resistance screening, and transgenic animal research. |
|---|---|
| 中文名称 |
G-418 溶液
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| 运输条件 |
Room temperature in continental US; may vary elsewhere. |
| 储存方式 |
Solution, -20°C, 1 year |
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