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HRP-Streptavidin
目录号 : KM32495

HRP-Streptavidin 是一种生物素结合型信号放大剂,可与生物素化抗 TSH 单克隆抗体紧密结合,构成免疫分析中的信号放大系统的一部分。 HRP-Streptavidin 通过将辣根过氧化物酶 (HRP) 与链霉亲和素 (Streptavidin) 共价偶联形成,结合了酶的高效催化能力与链霉亲和素对生物素的高亲和力,使其成为一种高效、灵敏的信号放大工具。HRP-Streptavidin 可用于 Western Blot,ELISA 或其他检测技术中。

规格 价格 是否有货 数量
500μg
In-stock
1mg
In-stock

Other Forms of Rapamycin:

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生物活性

Sulfonylurea Receptor HRP-Streptavidin is a biotin-binding signal amplifier designed to bind tightly with biotinylated anti-TSH monoclonal antibodies, thereby constituting a key component of the signal amplification system in immunoassays. HRP-Streptavidin is formed by the covalent coupling of Horseradish Peroxidase (HRP) with Streptavidin; it combines the highly efficient catalytic activity of the enzyme with the high affinity of Streptavidin for biotin, making it a potent and sensitive tool for signal amplification. HRP-Streptavidin is suitable for use in Western Blotting, ELISA, and other detection techniques.

体外研究

The HRP Streptavidin conjugate was successfully synthesized by the method of high iodate oxidation of HRP and coupling with streptavidin, forming a stable conjugate that can achieve signal amplification through biotin streptavidin binding.

HRP Streptavidin (1:500 dilution; 1 h) can achieve sensitive and accurate detection of 35 mer HBV DNA segments, with a detection limit of 3 pM, a working range of 6-100 pM, a sensitivity of 1.6 × 105 RLU/pM, and a coefficient of variation (CV) below 6%.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

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第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
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工作液浓度 mg/ml;

DMSO母液配制方法 mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,

体内配方配制方法μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。

配置后的溶液总体积

1. 首先保证母液是澄清的;
           2. 一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。