7-Hydroxycoumarinyl arachidonate (Synonyms:7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate)
目录号 : KM10645 CAS No. : 161180-11-6 纯度 : 98%

7-Hydroxycoumarinyl arachidonate (7-HCA) 是胞质磷脂酶 A2 (PLA2) 的荧光底物,也是单酰基甘油脂肪酶 (MAGL) 的荧光底物。MAGL 蛋白催化 7-Hydroxycoumarinyl arachidonate 的水解,生成花生四烯酸 (AA) 和高荧光的 7-羟基香豆素 (7-HC),7-HC 的释放可以用荧光计进行测量。

规格 价格 是否有货 数量
5mg
In-stock

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生物活性

7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; ). Release of 7-HC can be measured using a fluorometer.

体外研究

MAGL catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonate (7-HCA) into arachidonic acid (AA) and 7-HC. Release of 7-HC, which is fluorescent, is monitored over time with excitation (Ex) at 355 nm and emission (Em) at 460 nm.

分子式
C29H36O4
分子量
448.59
CAS号
161180-11-6
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

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体内配方配制方法μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。

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