Bohemine - CAS:189232-42-6

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Bohemine

目录号 : KM10524 CAS No. : 189232-42-6 纯度 : ≥98%

Data Sheet 产品使用指南

Bohemine 是一种嘌呤类似物，也是一种合成的选择性的 CDK 抑制剂，对 Cdk2/cyclin E，Cdk2/cyclin A 和 Cdk9/cyclin T1 的 IC₅₀ 分别为 4.6 μM，83 μM 和 2.7 μM。Bohemine 还抑制 ERK2，IC₅₀ 为 52 μM，对 CDK1，CDK4 和 CDK6 的抑制作用较小。Bohemine 具有广泛的抗癌活性。

规格	价格	是否有货	数量
5mg		In-stock

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生物活性

Bohemine is a purine analogue and is a synthetic and selective CDK inhibitor with IC₅₀s of 4.6 μM, 83 μM, and 2.7 μM for Cdk2/cyclin E, Cdk2/cyclin A, and Cdk9/cyclin T1, respectively. Bohemine also inhibits ERK2 with an IC₅₀ of 52 μM and has less inhibitory effect on CDK1, CDK4 and CDK6. Bohemine has a broad spectrum anti-cancer activities.

IC₅₀&Target

CDK2/cyclinE

4.6 μM (IC₅₀)

cdk2/cyclin A

83 μM (IC₅₀)

CDK9/cyclinT1

2.7 μM (IC₅₀)

ERK2

52 μM (IC₅₀)

体外研究

Bohemine (0-30 µM; 72 hours; ME-750 cells) treatment inhibits cell growth. Addition of Bohemine at concentrations in the range of 1-10 µM results in a short-term arrest of growth and of monoclonal antibody production. The short-term suppression of cell functions is followed by a significant temporary increase of specific growth rate and of specific production rate.
Hybridoma cells are retarded both at the G1/S boundary and at the G2/M boundary, depending on Bohemine (0-30 µM) concentration.
T-lymphoblastic cell line CEM is treated by Bohemine, five proteins are found to be downregulated, namely α-enolase, triosephosphate isomerase, initiation factor 5A, and α- and β-subunits of Rho GDP-dissociation inhibitor 1. These proteins play significant roles in glycolysis, proteosynthesis, and in cytoskeleton rearrangement.
Bohemine inhibits growth of human tumor cell lines with an IC₅₀ of 27 µM.

Cell Viability Assay

Cell Line:	Mouse hybridoma ME-750 cells
Concentration:	0 µM, 1 µM, 3 µM, 10 µM and 30 µM
Incubation Time:	72 hours
Result:	At 10 µM and 30 µM concentrations, the viable cell count was significantly lower with respect to control, i.e., 77% and 48%, respectively.

体内研究

Bohemine (50 mg/kg; intravenous injection; BALB/c mice) treatment shows C_max is 72,308 nM, observed clearance is 0.23 L/h and T_1/2 is 1.39 h.

Animal Model:	BALB/c mice bearing the colon 26 murine tumor
Dosage:	50 mg/kg
Administration:	Intravenous injection (Pharmacokinetic Analysis)
Result:	C_max is 72,308 nM, observed clearance is 0.23 L/h and T_1/2 is 1.39 h.

分子式

C₁₈H₂₄N₆O

分子量

340.42

CAS号

189232-42-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

纯度： ≥98%

Data Sheet

产品使用指南

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

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动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

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第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

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计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

Other Forms of Rapamycin:

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器