FOXO4-DRI - CAS:2460055-10-9

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FOXO4-DRI

目录号 : KP1519 CAS No. : 2460055-10-9

Data Sheet 产品使用指南

FOXO4-DRI 是一种细胞渗透性肽拮抗剂，可阻断 FOXO4 和 p53 的相互作用。FOXO4-DRI 是一种抗衰老多肽，可诱导衰老细胞凋亡 (apoptosis)。

规格	价格	是否有货	数量
1mg		In-stock
5mg		In-stock
10mg		In-stock

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生物活性

FOXO4-DRI is a cell-permeable peptide antagonist that blocks the interaction of FOXO4 and p53. FOXO4-DRI is a senolytic peptide that induces apoptosis of senescent cells.

体外研究

FOXO4-DRI (25 mM; 3 days) induces nuclear exclusion of active p53 and triggers apoptosis in senescent TM3 Leydig cells [1].
FOXO4-DRI (25 μM; 5 days) significantly reduced the senescence level of PDL9 cells [2].

Cell Viability Assay

Cell Line:

Senescent Leydig cells

Concentration:

25 mM

Incubation Time:

3 days

Result:

Reduced the viability of senescent as compared to normal TM3 Leydig cells.

Apoptosis Analysis

Cell Line:	Senescent Leydig cells
Concentration:	25 mM
Incubation Time:	3 days
Result:	The apoptosis rate increased from 10% to 27%.

Western Blot Analysis

Cell Line:	PDL9 cells
Concentration:	25 μM
Incubation Time:	5 days
Result:	Decreased the protein levels of representative senescent markers, including p16, p21, and p53.

RT-PCR

Cell Line:	PDL9 cells
Concentration:	25 μM
Incubation Time:	5 days
Result:	Enhanced SOX9 expression, and reduced MMP12 and MMP13 expression.

体内研究

FOXO4-DRI (5 mg/kg; i.p.; administered every other day for three doses) alleviates testosterone deficiency in naturally aging mice and improves the testicular microenvironment.

Animal Model:	Naturally aged male C57BL/6 mice (20-24 months old)
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection, every other day for three administrations
Result:	Increased serum testosterone levels. Increased levels of both 3β-HSD and CYP11A1. Decreased interstitial SA-β-gal activity and lowered levels of senescence-associated proteins p53, p21, and p16. Decreased the levels of IL-1β, IL-6 and TGF-β.

分子式

C₂₂₈H₃₈₈N₈₆O₆₄

分子量

5358.06

CAS号

2460055-10-9

中文名称

FOXO4-DRI

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

H2O : 100 mg/mL (18.66 mM; Need ultrasonic)

DMSO : 50 mg/mL (9.33 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.1866 mL	0.9332 mL	1.8663 mL
5 mM	0.0373 mL	0.1866 mL	0.3733 mL
10 mM	0.0187 mL	0.0933 mL	0.1866 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

Data Sheet

产品使用指南

[1]. Zhang C, et al. FOXO4-DRI alleviates age-related testosterone secretion insufficiency by targeting senescent Leydig cells in aged mice. Aging (Albany NY). 2020 Jan 20;12(2):1272-1284.

[2]. Huang Y, et al. Senolytic Peptide FOXO4-DRI Selectively Removes Senescent Cells From in vitro Expanded Human Chondrocytes. Front Bioeng Biotechnol. 2021 Apr 29;9:677576.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器