C16-PAF (Synonyms:PAF (C16))
目录号 : KM9946 CAS No. : 74389-68-7 纯度 : ≥98%

C16-PAF (PAF (C16)) 是一种磷脂介质,是血小板活化因子,也是 PAF G 蛋白偶联受体 (PAFR) 的配体。C16-PAF 表现出抗凋亡作用,并通过激活 PAFR 来抑制 caspase 依赖性死亡。C16-PAF 是有效的 MAPK 和 MEK/ERK 激活剂。C16-PAF 诱导血管通透性增加。

规格 价格 是否有货 数量
1mg
In-stock
5mg 询价 In-stock

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生物活性

C16-PAF (PAF (C16)), a phospholipid mediator, is a platelet-activating factor and ligand for PAF G-protein-coupled receptor (PAFR). C16-PAF exhibits anti-apoptotic effect and inhibits caspase-dependent death by activating the PAFR. C16-PAF is a potent MAPK and MEK/ERK activator. C16-PAF induces increased vascular permeability.

体外研究

C16-PAF (PAF (C16); 0.5-1.5 μM; for 24 hours) elicits significant concentration-dependent neuronal loss in PAFR but not PAFR cultures. C16-PAF (1 μM) elicits neuronal death in PAFR cells infected with EGFP alone.
C16-PAF (1 μM; for 24 hours) activates caspase 7 but not caspase 3 in PAFR neurons.
C16-PAF is synthesized by two distinct pathways; the remodeling pathway and the de novo synthesis pathway. C16-PAF acts by binding to a unique G-protein-coupled seven transmembrane receptor.
C16-PAF (1-25 μg/ml; 6, 12, 24 h) inhibits M. smegmatis and M. bovis BCG growth in a time-dependent manner.

Cell Viability Assay

Cell Line: Cerebellar granule neurons (CGNs) from PAFR and PAFR mice
Concentration: 0.5-1.5 μM
Incubation Time: 24 hours
Result: Elicited significant concentration-dependent neuronal loss in PAFR but not PAFR cultures in serum-free media.

Western Blot Analysis

Cell Line: CGNs
Concentration: 1 μM
Incubation Time: 24 hours
Result: Activated caspase 7 but not caspase 3 in PAFR neurons.
分子式
C26H54NO7P
分子量
523.68
CAS号
74389-68-7
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性数据
In Vitro: 

DMSO : 50 mg/mL (95.48 mM; Need ultrasonic)

H2O : 33.33 mg/mL (63.65 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9096 mL 9.5478 mL 19.0956 mL
5 mM 0.3819 mL 1.9096 mL 3.8191 mL
10 mM 0.1910 mL 0.9548 mL 1.9096 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (4.77 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (4.77 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.77 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

科研文献
The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
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