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Deoxyribonuclease (Synonyms:脱氧核糖核酸酶;DNase I (RNase free);DNase (RNase free))
目录号 : KP12654 CAS No. : 9003-98-9

Deoxyribonuclease I digests single- and double-stranded DNA into a mixture of mono- and oligonucleotides with 5′ phosphate and 3′ hydroxyl termini. This catalytic activity is dependent on divalent ions. In the presence of Mg2+, deoxyribonuclease I randomly and independently hydrolyzes each strand of double-stranded DNA. In the presence of Mn2+, both strands can be cleaved.
Used in molecular biology applications for DNA removal during RNA purification, for preparing DNA for nick translation, and for analyzing DNA-protein interactions by footprinting. RNAase-free deoxyribonuclease I solutions from bovine pancreas have been used to digest DNA in a variety of samples.

 

规格 价格 是否有货 数量
50mg
In-stock
立即试用
100mg
In-stock
250mg 询价 In-stock
500mg 询价 In-stock
1g 询价 In-stock

Other Forms of Rapamycin:

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分子式
C12H22N4O6
分子量
318.33
CAS号
9003-98-9
中文名称
脱氧核糖核酸酶
运输条件

Room temperature in continental US; may vary elsewhere. 

储存方式
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
暂无相关参考文献
The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
动物实验计算换算器
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系客服为您提供正确的澄清溶液配方)
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+
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计算结果:

工作液浓度 mg/ml;

DMSO母液配制方法 mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,

体内配方配制方法μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。

配置后的溶液总体积

1. 首先保证母液是澄清的;
           2. 一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。