eIF4A3-IN-2 is a highly selective and noncompetitive eukaryotic initiation factor 4A-3 (eIF4A3) inhibitor with an IC50 of 110 nM.
体外研究
eIF4A3-IN-2 (compound 2) binds to the allosteric region in eIF4A3 and inhibits in vitro ATPase, helicase, and cellular nonsense-mediated RNA decay (NMD) activities.
Apoptosis Analysis
Cell Line:
HEK293T cells (transfected with the NMD reporter) luciferase assay
Concentration:
0.3, 1, 3, or 10 μM
Incubation Time:
3 or 6 h
Result:
eIF4A3-IN-2 induces an approximately 3.2-fold increase in luciferase activity, indicating that NMD is inhibited by compound 2.
分子式
C25H19Br2ClN4O2
分子量
602.70
CAS号
2095677-20-4
运输条件
Room temperature in continental US; may vary elsewhere.