12(S)-HETE - CAS:54397-83-0

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12(S)-HETE

目录号 : KCM14209 CAS No. : 54397-83-0 纯度 : ≥95%

Data Sheet 产品使用指南

12(S)-HETE is the predominant lipoxygenase product of mammalian platelets. It enhances tumor cell adhesion to endothelial cells, fibronectin, and the subendothelial matrix at 0.1 µM.12(S)-HETE MaxSpec standard is a quantitative grade standard of 12(S)-HETE that has been prepared specifically for mass spectrometry or any application where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This 12(S)-HETE MaxSpec standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

规格	价格	是否有货	数量
25μg		In-stock
50μg		In-stock
100μg		In-stock

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生物活性

12(S)-HETE is a metabolite of Arachidonic acid. 12(S)-HETE promotes ERK and P38 MAPK phosphorylation, stimulates DNA synthesis and cell proliferation in cancer cells. 12(S)-HETE facilitates wound healing of injured murine-lung-derived microvascular endothelial cell monolayers. 12(S)-HETE can be used for the research of pancreatic cancer.

体外研究

12 (S) - HETE (1-100 nM; 2h) can stimulate DNA synthesis of human pancreatic cancer cells PANC-1 and HPAF in a concentration dependent manner.
12 (S) - HETE (24-96 h) can promote the proliferation of human pancreatic cancer cells PANC-1 and HPAF in a time-dependent manner.
12 (S) - HETE (100 nM; 1-60 min) can time-dependently induce tyrosine phosphorylation of various cell proteins in human pancreatic cancer cells PANC-1 and HPAF.
12 (S) - HETE (100 nM; 1 min-24 h) can activate ERK1/2 phosphorylation in human pancreatic cancer cell lines PANC-1 and HPAF in a time-dependent manner.
12 (S) - HETE (100 nM; 1-60 min) can activate phosphorylation of P38 MAPK in human pancreatic cancer cell lines PANC-1 and HPAF in a time-dependent manner, but this activation has nothing to do with its mitogenic effect.
12 (S) - HETE (100 nM; 1-60 min) does not induce JNK/SAPK phosphorylation in human pancreatic cancer cells PANC-1 and HPAF.
12 (S) - HETE (0.1 μM; 8 days) can promote accelerated wound healing of damaged CD clone 4 mouse lung derived microvascular endothelial cell monolayers.
12 (S) - HETE (0.01-10 μM; 1-4 days) dose dependently promotes the proliferation of pulmonary derived microvascular endothelial cells in CD clone 4 mice.
12 (S) - HETE (0.1 μM; 24 h) can increase the DNA synthesis level of lung derived microvascular endothelial cells in CD clone 4 mice with growth arrest by more than 4 times.

Western Blot Analysis

Cell Line:	PANC-1, HPAF
Concentration:	100 nM
Incubation Time:	1 min, 5 min, 15 min, 30 min, 60 min
Result:	Stimulated tyrosine phosphorylation of multiple cellular proteins of varying molecular sizes in a time-dependent manner, with strong phosphorylation evident within 1 min and maintained throughout 60 min.

Western Blot Analysis

Cell Line:	PANC-1, HPAF
Concentration:	100 nM
Incubation Time:	1 min, 5 min, 10 min, 15 min, 30 min, 60 min, 3 h, 6 h, 24 h
Result:	Induced time-dependent activation of ERK1/2 in PANC-1 and HPAF cells.

Cell Proliferation Assay

Cell Line:	murine-lung-derived microvascular endothelial cells (CD clone 4)
Concentration:	0.001, 0.01, 0.1,1, 10 μM
Incubation Time:	1-4 days
Result:	Promoted CD clone 4 cell growth in a dose-dependent manner.

分子式

C₂₀H₃₂O₃

分子量

320.47

CAS号

54397-83-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

纯度： ≥95%

Data Sheet

产品使用指南

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Other Forms of Rapamycin:

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