Chlorin e6 - CAS:19660-77-6

您当前的位置：

Chlorin e6 (Synonyms:二氢卟吩 E6; Ce6)

目录号 : KCM12322 CAS No. : 19660-77-6 纯度 : ≥98%

Data Sheet SDS 产品使用指南

Chlorin e6 (Ce6) is a photosensitizer. It is active against S. aureus, P. aeruginosa, E. coli, and S. typhimurium when used at a concentration of 20 µM in combination with photodynamic therapy (PDT). Ce6 (5 and 10 mg/kg) induces tumor regression when combined with PDT in several sarcoma rat xenograft models.

规格	价格	是否有货
50mg		In-stock
100mg		In-stock
250mg	询价	In-stock
500mg	询价	In-stock

加入购物车大包装询价

Other Forms of Rapamycin:

Sodium Selenite In-stock
4"-Isovalerylspiramycin I In-stock
Higenamine-d4 hydrochloride In-stock
Adebrelimab In-stock
Tigatuzumab In-stock

KKL Med 的所有产品和服务仅用于科学研究，不能被用于人体，兽医，我们也不向个人提供产品和服务。

生物活性

Chlorin e6 is a photosensitizer and has strong absorption peaks at wavelength of 402 and 662 nm, as well as exhibiting intense fluorescence at 668 nm. Chlorin e6 has antimicrobial efficacy and anticancer activity. Chlorin e6 induces cell apoptosis via caspase-3 activation and can be used for the research of cancer.

体外研究

Chlorone6 (100 μg/mL, 24 h) showed no toxicity to HT-29 cells under 635 nm laser irradiation.
Chlorin e6 (5-10 μM, 3 h) induces apoptosis of pancreatic cancer cells by activating caspase-3 under 660 nm near-infrared laser irradiation, thereby inhibiting tumor growth of AsPC-1 and MIA PaCa-2 cells.

Western Blot Analysis

Cell Line:

AsPC-1 and MIA PaCa-2 Cells

Concentration:

5 μM, 10 μM

Incubation Time:

3 hour

Result:

Significantly reduced Bcl-2 protein expression while increasing apoptotic molecule expression of Bax.
Activated caspase-3 through its cleavage and upregulated the expression of cleaved PARP-1 in a dose-dependent manner.

体内研究

Chlorone6 (2.5 mg/kg, intravenous injection, single dose) exhibited anti-tumor activity in B16F10 melanoma cell and PANC02 pancreatic cell xenograft mouse models after 8 min and 20 s of red light irradiation at 100 J/cm2.
Chlorone6 (2.5 mg/kg, intravenous injection, single dose) can inhibit tumor growth in dogs under 660 nm near-infrared laser irradiation.

Animal Model:	B16F10 melanoma cells xenograft mouse, PANC02 pancreatic cells xenograft mouse
Dosage:	2.5 mg/kg
Administration:	Intravenous injection (i.v.)
Result:	Significantly reduced tumor volume.

分子式

C₃₄H₃₆N₄O₆

分子量

596.67

CAS号

19660-77-6

中文名称

二氢卟吩 E6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 20 mg/mL (33.52 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.6760 mL	8.3798 mL	16.7597 mL
5 mM	0.3352 mL	1.6760 mL	3.3519 mL
10 mM	0.1676 mL	0.8380 mL	1.6760 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO → 90% (20% SBE-β-CD in saline)

Solubility: 2.08 mg/mL (3.49 mM); Suspended solution; Need ultrasonic

此⽅案可获得 2.08 mg/mL (3.49 mM) 的均匀悬浊液，悬浊液可⽤于⼝服和腹腔注射。

以 1 mL ⼯作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。

纯度： ≥98%

Data Sheet

SDS

产品使用指南

Karuppusamy S, et al. Nanoengineered chlorin e6 conjugated with hydrogel for photodynamic therapy on cancer [J]. Colloids and Surfaces B: Biointerfaces, 2019, 181: 778-788.

Shrestha R, et al. Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors [J]. Life, 2022, 12(12): 2102.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

Other Forms of Rapamycin:

KKL 顾客使用本产品发表的 0 篇科研文献

The molarity calculator equation

The dilution calculator equation

动物实验计算换算器