Boscalid - CAS:188425-85-6

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Boscalid (Synonyms:啶酰菌胺)

目录号 : KCM12223 CAS No. : 188425-85-6 纯度 : ≥95%

Data Sheet SDS 产品使用指南

Boscalid is a broad-spectrum carboxamide fungicide that inhibits fungal respiration by binding to the ubiquinone site of mitochondrial complex II/succinate dehydrogenase. It suppresses mycelial growth of S. minor by 87 to 100% and of S. sclerotiorum by 77 to 100% when used at a concentration of 1 µg/ml. In field studies, boscalid applied at 5.6 µg/cm provides 55.5 and 30.4% disease control for lettuce drop caused by S. minor and S. sclerotiorum, respectively. It decreases cell viability of mouse primary cortical neurons following long-term exposure but is not cytotoxic (LC = >100 µM for acute and continuous exposure). Formulations containing boscalid have been used in agriculture to prevent fungal growth on crops.

规格	价格	是否有货	数量
10 mM * 1 mL in DMSO		In-stock
5g		In-stock
10g		In-stock
25g		In-stock
50g		In-stock

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生物活性

Boscalid is a succinate dehydrogenase (SDHI) inhibitor with antifungal activity. Boscalid binds to the ubiquinone-binding site of fungal mitochondrial complex II, blocks ATP production and aerobic respiration, exhibits good control efficacy against a variety of plant fungal diseases including gray mold, sclerotinia rot and powdery mildew, and is widely used for disease control in agriculture. Boscalid induces apoptosis, altered lipid metabolism, mitochondrial dysfunction, respiratory impairment, oxidative stress, ROS accumulation and neurodevelopmental disorders in zebrafish. Boscalid reduces foraging ability, shortens median death time and causes chronic toxicity in exposed honeybees. Boscalid also possesses genotoxicity, cytotoxicity, elevated mitochondrial superoxide levels and early-stage apoptosis.

IC₅₀&Target

succinate dehydrogenase (SDH)

体内研究

Boscalid (5-25 mg/L; 6-48 hpf (embryos); 24 hours (6 dpf larvae) can induce concentration dependent neurodevelopmental toxicity and oxidative stress in zebrafish (Danio rerio) embryos and juveniles.

Animal Model:	Tg(elavl3: EGFP) transgenic line, AB strain
Dosage:	5 mg/L; 15 mg/L; 25 mg/L
Administration:	waterborne; 6 to 48 hpf (embryos); 24 h (6 dpf larvae)
Result:	Observed obscure brain regional division, blurred ventricles, malformed eyes, and decreased spontaneous tail coiling frequency at 24 hpf in embryos. Detected enlarged cerebral ventricles, reduced pigmentation, decreased neonatal neurons, defective development around ventricles, increased brain intercellular space, and concentrated nuclei at 48 hpf in embryos. Measured increased Na⁺K⁺-ATPase, Ca²⁺Mg²⁺-ATPase, and total ATPase activities; increased MDA content; accumulated ROS in head and heart; increased CAT activity; decreased SOD activity; decreased tyrosinase activity in embryos. Recorded decreased expression of tfap2a, tfap2e, sox10, gfap, gap43; increased then decreased expression of wnt1, mitfa, pax3a; increased expression of sliva, oca2, nestin, neurogenin1 in embryos. Found increased central area motion proportion, increased angular velocity and sinuosity; decreased total distance, average speed, movement duration, mobile/high mobile frequency and duration in larvae.

Animal Model:	Five-week-old female C.B-17/lcr-scid/scidJcl mice (bearing HeLa cells)
Dosage:	1 mg/kg
Administration:	Intravenous injection; twice a week for 4 weeks
Result:	The growth inhibition rates in HeLa tumors was 49% compared to the control.

分子式

C₁₈H₁₂Cl₂N₂O

分子量

343.21

CAS号

188425-85-6

中文名称

啶酰菌胺

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 100 mg/mL (291.37 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9137 mL	14.5683 mL	29.1367 mL
5 mM	0.5827 mL	2.9137 mL	5.8273 mL
10 mM	0.2914 mL	1.4568 mL	2.9137 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂：10% DMSO → 40% PEG300 → 5% Tween-80 → 45% saline

Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.28 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂：10% DMSO → 90% corn oil

Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.28 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

纯度： ≥95%

Data Sheet

SDS

产品使用指南

Wang H, et al. Characterization of boscalid-induced oxidative stress and neurodevelopmental toxicity in zebrafish embryos. Chemosphere. 2020;238:124753.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器