22(R)-hydroxy Cholesterol - CAS:17954-98-2

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22(R)-hydroxy Cholesterol (Synonyms:22(R)-羟基胆固醇;Narthesterol)

目录号 : KCM12060 CAS No. : 17954-98-2 纯度 : ≥98%

Data Sheet 产品使用指南

The liver X receptors (LXRα and LXRβ) are nuclear hormone receptors whose native ligands are oxysterols. LXRs regulate the oxysterol-induced expression of cholesterol 7α-hydroxylase, the rate limiting enzyme of classic bile acid synthesis. 22(R)-hydroxy Cholesterol is an endogenous agonist for LXRs that activates LXRα with an EC value of 325 nM. 22(R)-hydroxy Cholesterol, acting through LXR heterodimerized with the retinoid X receptor, induces the expression of the ABCA1 reverse cholesterol transporter. This activity increases the efflux of cholesterol from enterocytes and thus inhibits the overall absorption of cholesterol. 22(R)-hydroxy Cholesterol can be used as a substrate to monitor cholesterol transport or as an endogenous positive control for testing LXR agonists which have potential as therapeutic agents for the treatment of atherosclerosis.

规格	价格	是否有货	数量
1mg		In-stock
5mg		In-stock
10mg		In-stock

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生物活性

22(R)-hydroxy Cholesterol is a natural oxysterol, acting as a LXRα agonist that inhibits cancer cell proliferation.

22 (R)-Hydroxycholesterol activates LXRα-mediated upregulation of downstream ABCA1 expression, enhances apolipoprotein-dependent cholesterol efflux, reduces intracellular cholesterol content, and decreases the production of β-amyloid peptide.

22 (R)-Hydroxycholesterol induces the differentiation of human mesenchymal stem cells into functional dopaminergic neurons.

22 (R)-Hydroxycholesterol is applicable to research related to cancer, Alzheimer's disease, and Parkinson's disease.

IC₅₀&Target

LXRα

体外研究

22(R)-hydroxy Cholesterol (10-20 μM; 24 h) combined with 9-cis Retinoic acid can upregulate the expression of ABCA1, increase cholesterol efflux, regulate APP processing, and reduce A β secretion in H4APPsw and CHOAPPsw cells.

22(R)-hydroxy Cholesterol (22-HC) (2 μM; 14 days) can induce human BM MSCs, AD MSCs, and DP MSCs to differentiate into dopaminergic neurons. Among them, DP MSCs have the highest differentiation efficiency (MAP2 positive cells account for 80.20%), and the upregulation of dopaminergic markers and functional characteristics is the most significant.
22(R)-hydroxy Cholesterol (30 nM-3 μM) can activate LXR α in transfected HEK293 cells, with significant activation observed at a concentration of 1 μM and higher activation levels at a concentration of 3 μM.
22(R)-hydroxy Cholesterol (1-12 μM; 96 h) can dose dependently inhibit the proliferation of human prostate cancer cell line LNCaP, with EC50 values ranging from 4.7 μM to 6.3 μM; It can dose dependently inhibit the proliferation of various human cancer cell lines, with EC50 values ranging from 7.0 μM to 10.1 μM.

Cell Differentiation Assay

Cell Line:	human bone marrow-derived mesenchymal stem cells (BM-MSCs), adipose tissue-derived mesenchymal stem cells (AD-MSCs), dental pulp-derived mesenchymal stem cells (DP-MSCs)
Concentration:	2 μM
Incubation Time:	14 days
Result:	Induced dopaminergic neuronal differentiation of human BM-MSCs, AD-MSCs, and DP-MSCs, with DP-MSCs exhibiting the highest differentiation efficiency (80.20% MAP2-positive cells) and most robust upregulation of dopaminergic markers and functional traits.

Cell Proliferation Assay

Cell Line:

LNCaP human prostate cancer cell sublines (104-S, 104-R1, 104-R2, R1Ad, CDXR-3, IS-3)

Concentration:

1 μM, 2 μM, 4 μM, 8 μM, 12 μM

Incubation Time:

96 hours

Result:

Dose-dependently suppressed the proliferation of all tested cell sublines.
Achieved half-maximal effective concentration (EC₅₀) values for growth inhibition of 4.7 μM (104-R2), 5.2 μM (CDXR-3), 5.7 μM (104-S), 5.8 μM (104-R1), 5.9 μM (IS-3), and 6.3 μM (R1Ad).

Cell Proliferation Assay

Cell Line:

multiple human cancer cell lines (DU-145, SCC13, MCF-7, Saos-2, HeLa, PC-3, MDA-MB-435, H1299, A431, HepG2)

Concentration:

1 μM, 2 μM, 4 μM, 8 μM, 12 μM

Incubation Time:

96 hours

Result:

Dose-dependently suppressed the proliferation of all tested cell lines, with greater suppression observed at 8 μM or higher concentrations.
Achieved half-maximal effective concentration (EC₅₀) values for growth inhibition of 7.0 μM (DU-145), 7.2 μM (SCC13), 7.4 μM (MCF-7), 7.6 μM (Saos-2), 7.7 μM (HeLa), 7.7 μM (PC-3), 8.0 μM (MDA-MB-435), 8.4 μM (H1299), 8.6 μM (A431), and 10.1 μM (HepG2).

分子式

C₂₇H₄₆O₂

分子量

402.65

CAS号

17954-98-2

中文名称

22(R)-羟基胆固醇

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

Ethanol : ≥ 100 mg/mL (248.35 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4835 mL	12.4177 mL	24.8355 mL
5 mM	0.4967 mL	2.4835 mL	4.9671 mL
10 mM	0.2484 mL	1.2418 mL	2.4835 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% EtOH → 90% corn oil

Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.21 mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900 μL ⽟⽶油中，混合均匀。

纯度： ≥98%

Data Sheet

产品使用指南

Koldamova RP, et al. 22R-hydroxycholesterol and 9-cis-retinoic acid induce ATP-binding cassette transporter A1 expression and cholesterol efflux in brain cells and decrease amyloid beta secretion. J Biol Chem. 2003 Apr 11;278(15):13244-56.

Chuu CP, et al. Antiproliferative effect of LXR agonists T0901317 and 22(R)-hydroxycholesterol on multiple human cancer cell lines. Anticancer research. 2010 Sep;30(9):3643-8.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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The molarity calculator equation

The dilution calculator equation

动物实验计算换算器