BODIPY 493/503 is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells. BODIPY 493/503 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow cytometry. It displays excitation/emission maxima of 493/503 nm, respectively, and can be used for live and fixed cell applications.
BODIPY493/503 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 493/503 nm.
分子式
C14H17BF2N2
分子量
262.11
CAS号
121207-31-6
中文名称
4,4-二氟-1,3,5,7,8-五甲基-4-硼-3A,4A-二氮杂-S-茚烯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
2-8°C, sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:
DMF: 5.56 mg/mL (21.21 mM; ultrasonic and warming and heat to 60°C)
DMSO : 5 mg/mL (19.08 mM; ultrasonic and warming and heat to 60°C)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
3.8152 mL
19.0760 mL
38.1519 mL
5 mM
0.7630 mL
3.8152 mL
7.6304 mL
10 mM
0.3815 mL
1.9076 mL
3.8152 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。