c-di-AMP sodium
目录号 : KM4631 纯度 : 98%

c-di-AMP (Cyclic diadenylate) sodium 是 STING 激动剂,其与 STING 结合,从而激活 TBK3-IRF3 信号传导途径,随后引发 I 型 IFN 和 TNF 产生。c-di-AMP sodium 是一种细菌第二信使,主要在革兰氏阳性细菌中,可调节细胞生长,存活以及毒力。c-di-AMP (Cyclic diadenylate) 也调节宿主免疫反应。c-di-AMP sodium 可作为一种有效的粘膜佐剂,刺激体液和细胞反应

规格 价格 是否有货 数量
500μg
In-stock
1mg
In-stock
5mg
In-stock
10mg
In-stock
25mg
In-stock
50mg 询价 In-stock
100mg 询价 In-stock

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生物活性

c-di-AMP (Cyclic diadenylate) sodium is a STING agonist, which binds to the transmembrane protein STING thereby activating the TBK3-IRF3 signaling pathway, subsequently triggering the production of type I IFN and TNF. c-di-AMP sodium is also a bacterial second messenger, which regulates cell growth, survival, and virulence, primarily within Gram-positive bacteria, and also regulates host immune response. c-di-AMP sodium acts as a potent mucosal adjuvant stimulating both humoral and cellular responses.

体外研究

c-di-AMP (Cyclic diadenylate) sodium signaling is a central factor in many Gram-positive bacteria regulating cell wall synthesis, potassium ion channels, DNA repair, and biofilm formation. c-di-AMP sodium is also essential for cell growth, survival, and virulence of several well-known human pathogenic bacteria including S. aureus, L. monocytogenes, S. pyogenes, and Mycobacterium spp.
c-di-AMP sodium combines with model antigens, such as OVA or β-Gal, acts as a potent mucosal adjuvant stimulating both humoral and cellular responses.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-80°C, protect from light, stored under nitrogen

溶解性数据
In Vitro: 

DMSO : 270 mg/mL (Need ultrasonic)

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 6.75 mg/mL (Infinity mM); Clear solution

    此方案可获得 ≥ 6.75 mg/mL (Infinity mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 67.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 6.75 mg/mL (Infinity mM); Clear solution

    此方案可获得 ≥ 6.75 mg/mL (Infinity mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 67.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 6.75 mg/mL (Infinity mM); Clear solution

    此方案可获得 ≥ 6.75 mg/mL (Infinity mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 67.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
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