Butaprost - CAS:69685-22-9

您当前的位置：

Butaprost

目录号 : KM3875 CAS No. : 69685-22-9 纯度 : 98%

Data Sheet SDS 产品使用指南

Butaprost 是一种选择性前列腺素 E 受体 (EP2) 激动剂，对鼠类 EP2 受体的 EC₅₀ 值为 33 nM，K_i 值为 2.4 μM。Butaprost 对鼠类 EP1，EP3 和 EP4 受体的活性较低。Butaprost 通过阻碍 TGF-β/Smad2 信号传导来减轻纤维化。

规格	价格	是否有货	数量
5mg		In-stock

加入购物车大包装询价

Other Forms of Rapamycin:

Dydrogesterone-D6 In-stock
Prostaglandin E1-d4 In-stock
Norcholic acid In-stock
Dicyclohexyl phthalate-3,4,5,6-d4 In-stock
Mebhydrolin napadisylate In-stock

KKL Med 的所有产品和服务仅用于科学研究，不能被用于人体，兽医，我们也不向个人提供产品和服务。

生物活性

Butaprost is a selective prostaglandin E receptor (EP2) agonist with an EC₅₀ of 33 nM and a K_i of 2.4 μM for murine EP2 receptor. Butaprost is less activity against murine EP1, EP3 and EP4 receptors. Butaprost attenuates fibrosis by hampering TGF-β/Smad2 signalling.

IC₅₀&Target

m-EP₂ receptor

2400 nM (Ki)

m-EP₂ receptor

33 nM (EC₅₀)

体外研究

Butaprost (1-100 nM; 0.5-24 hours) induces about a five-fold upregulation of Nur77 mRNA expression in hEP2-HEK293/EBNA cells in a dose- and time-dependent. Butaprost upregulated Nur77 gene expression through the PKC pathway.
Butaprost (50 μM; 24 hours) reduces TGF-β-induced fibronectin (FN) expression, Smad2 phosphorylation and epithelial-mesenchymal transition in Madin-Darby Canine Kidney (MDCK) cells.

Western Blot Analysis

Cell Line:	HEK 293/EBNA cells stably expressing the human EP2 receptor (hEP2-HEK 293/EBNA cells)
Concentration:	1 nM, 10 nM, 100 nM
Incubation Time:	0.5 hours, 1 hours, 6 hours, 24 hours
Result:	Induced about a five-fold upregulation of Nur77 mRNA expression in hEP2-HEK293/EBNA cells.

体内研究

Butaprost (1-4 mg/kg; intraperitoneal injection; twice daily; for 7 days) treatment attenuates the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery, as illustrated by a reduction in the gene and protein expression of α-smooth muscle actin, fibronectin and collagen 1A1.

Animal Model:	Male C57BL/6 mice (8 weeks of age; 21 g) bearing unilateral ureteral obstruction surgery
Dosage:	1 mg/kg, 2 mg/kg, 4 mg/kg
Administration:	Intraperitoneal injection; twice daily; for 7 days
Result:	Attenuated the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery.

分子式

C₂₄H₄₀O₅

分子量

408.57

CAS号

69685-22-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Solution, -20°C, 2 years

纯度： 98%

Data Sheet

SDS

产品使用指南

暂无相关参考文献

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

重置计算

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

重置计算

动物实验计算换算器

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% ddH₂O

% DMSO

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

配置后的溶液总体积：

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

Other Forms of Rapamycin:

KKL 顾客使用本产品发表的 0 篇科研文献

The molarity calculator equation

The dilution calculator equation

动物实验计算换算器