FM-red
目录号 : KM12027 纯度 : 98%

FM-red (PSH-red) 是一种发红光且对环境敏感的探针,用于选择性检测和标记蛋白硫醇。FM-red 可用于在活细胞和体内蛋白质巯基的成像。 FM-red 也可用于测量硫氧还蛋白 (Trx) 的氧化还原状态。

规格 价格 是否有货 数量
5mg
In-stock
10mg
In-stock

Other Forms of Rapamycin:

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生物活性

FM-red (PSH-red) is a red-emitting and environment-sensitive probe for selectively detecting and labeling protein thiols. FM-red can be used to image protein sulfhydryl groups in live cells and in vivo. FM-red also could be used to measure of the redox states of thioredoxin (Trx).

体外研究

FM-red (10 μM; 30 min) is suitable for imaging protein thiols in HeLa cells.
FM-red (20 μM; 48 h) has no significant cytotoxicity in HeLa cells.
FM-red possesses a long emission wavelength (~655 nm) and fast response (~10 min) by binding to protein thiols.
FM-red exhibits time- and concentration-dependent fluorescence response to bovine serum albumin (BSA).

体内研究

FM-red (10 μM; 30 min) could be used to image protein thiols in zebrafishes.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

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第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
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工作液浓度 mg/ml;

DMSO母液配制方法 mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,

体内配方配制方法μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。

配置后的溶液总体积

1. 首先保证母液是澄清的;
           2. 一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。