Mito-TRFS, the first off-on probe, is used to image the mitochondrial thioredoxin reductase (TrxR2) in live cells.
体外研究
Mito-TRFS (10 μM) has the maximal absorption at ~375 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4) at 37 °C.
Mito-TRFS itself has a strong emission at ~480 nm when excited at 375 nm in TE buffer (φ=0.34), but has weak emission when excited at 438 nm.
Immunofluorescence.
Cell Line:
HeLa cells.
Concentration:
1 μM.
Incubation Time:
2 h.
Result:
Mito-TRFS is localized predominantly in the mitochondria of live cells.
分子式
C38H34IN2O5PS2
分子量
820.70
CAS号
1859102-62-7
运输条件
Room temperature in continental US; may vary elsewhere.