CRM197 是一种白喉毒素的无毒突变体，甘氨酸被谷氨酸单一取代。 CRM197 是一种定义明确的蛋白质，可以作为多糖和多聚体的载体，使它们更具免疫原性。 CRM197 被用作许多已批准的结合疫苗的载体蛋白，用于肺炎链球菌、b型流感嗜血杆菌和脑膜炎奈瑟菌引起的疾病。重组 CRM197 是一种58.4kDa的球状蛋白，含有535个氨基酸。
| 储存方式 |
Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
|---|---|
| 研究背景 |
CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of glycine with glutamic acid. CRM197 is a well-defined protein and functions as a carrier for polysaccharides and haptens making them more immunogenic. CRM197 is utilized as a carrier protein in a number of approved conjugate vaccines for diseases caused by Streptococcus pneumoniae, Haemophilus influenzae b and Neisseria meningitidis. Recombinant CRM197 is a 58.4kDa globular protein containing 535 amino acids.
|
| 表达系统 |
Expressed in E. coli.
|
| Accession号 |
AMV91693 Gly2-Ser536
|
| 预测分子量 |
Approximately 58.4 kDa, a single non-glycosylated polypeptide chain containing 535 amino acids
|
| 性状 |
Sterile filtered White lyophilized (freeze-dried) powder.
|
| 保存溶液 |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 0.025% (v/v) Tween-80 pH7.4.
|
| 纯度 |
> 96% by SDS-PAGE or HPLC, dimers and high molecular weight impurities: <4% by SEC-HPLC.
|
| 内毒素含量 |
< 0.01 EU/μg of rCRM197 protein as determined by LAL method.
|
关注我们
获得KKL MED 最新资讯
咨询热线：
400-881-9290
Data Sheet