您当前的位置:
Human APOA1 Recombinant Protein (N-His)
目录号 : KP14030 纯度 : >90% by SDS-PAGE

Recombinant Human APOA1 Protein, N-His expressed in E. coli, spanning Arg19-Gln267. Purity: >90% by SDS-PAGE.

Highlights

His-Tagged — N-terminal 6×His tag for IMAC purification.

E. coli Expression — High-yield, cost-effective production.

High Purity — >90% purity verified by SDS-PAGE.

规格 价格 是否有货 数量
50µg
In-stock
100µg
In-stock
KKL Med 的所有产品和服务仅用于科学研究,不能被用于人体,兽医,我们也不向个人提供产品和服务。
生物活性

Recombinant Human APOA1 Protein, N-His expressed in E. coli, spanning Arg19-Gln267. Purity: >90% by SDS-PAGE.

Highlights

His-Tagged — N-terminal 6×His tag for IMAC purification.

E. coli Expression — High-yield, cost-effective production.

High Purity — >90% purity verified by SDS-PAGE.

中文名称
ApoA-I, ProapoA-I, Apolipoprotein A1, Apo-AI, Apolipoprotein A-I, APOA1, Apolipoprotein A-I(1-242)
运输条件

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

储存方式

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

研究背景
Apolipoprotein A-I (APOA1) is a ~30 kDa protein. Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
种属
Homo sapiens (Human)
表达系统
E. coli
Accession号
P02647
蛋白长度
Arg19-Gln267
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
预测分子量
31.25 kDa
性状
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
纯度
>90% as determined by SDS-PAGE.

纯度: >90% by SDS-PAGE

暂无相关参考文献
The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
动物实验计算换算器
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系客服为您提供正确的澄清溶液配方)
+
+
+

计算结果:

工作液浓度 mg/ml;

DMSO母液配制方法 mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,

体内配方配制方法μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。

配置后的溶液总体积

1. 首先保证母液是澄清的;
           2. 一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。